Apparatus and methods for controlling fluid flow in microchannels

• US 5,632,876 A
• Filed: 06/06/1995
• Issued: 05/27/1997
• Est. Priority Date: 06/06/1995
• Status: Expired due to Term

• Current Assignee: Sarnoff Corporation (SRI International, Inc.)
• Original Assignee: David Sarnoff Research Center, Inc.
• Inventors: Zanzucchi, Peter J., McBride, Sterling E., Cherukuri, Satyam C., Burton, Charlotte A.
• Primary Examiner(s): Phasge, Arun S.
• Application Number:
  US08/469,238
• Time in Patent Office: 721 Days
• Field of Search: 204/180.1, 204/182.1, 204/299 R, 204/600, 417/48, 417/49, 417/50, 417/53
• US Class Current: 204/600
• CPC Class Codes: B01F 33/3032 using magneto-hydrodynamic [MHD] phenomena to mix or move the fluids
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00353 Pumps
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/0068 Means for controlling the apparatus of the process
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 4/02 for feeding measured , i.e. prescribed quantities of reagents
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0418 electro-osmotic flow [EOF]
• CPC Class Codes: B01L 2400/06 Valves, specific forms thereof
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence

Abstract

By combining electro-osmotic and electrohydrodynamic pumps in a microchannel, both polar and non-polar fluids can be moved along said channel. The pumps can be made from pairs of wire electrodes inserted into openings in the channel and connected to a source of a pulsed DC power. By reversing the voltages on alternate pairs of pumps, fluid flow can be reversed, thereby acting as a gate or valve. By using digital drivers, for example shift registers that can apply a signal to a switching device connected to an electrode by means of enabling and latch signals to an AND gate, control of flow in individual channels in an array of channels can be had with a high degree of integration, and provide for ready manufacturability.

Claims

1. A pumping device in a microchannel comprising electrodes situated alone the length of the microchannel, two of which are separated by a first length of microchannel and can be operated together to form an electrohydrodynamic pump and two of which are separated by a second length of microchannel and can be operated together to form an electro-osmotic pump, wherein the second length is greater than the first length.

2. The pumping device according to claim 1, further comprising additional electrohydrodynamic or electroosmotic pumps present in said microchannel.

3. The pumping device according to claim 2, comprising a first electrohydrodynamic or electro-osmotic pump located in the microchannel that can be operated with a first voltage and a second electrohydrodynamic or electro-osmotic pump that can be operated with a second voltage of opposite polarity from the first voltage, wherein such operation of the first and second pumps is effective to stop the flow of fluid in the microchannel.

4. The pumping device of claim 3, wherein the first and second pumps are either both electrohydrodynamic pumps or are both electro-osmotic pumps.

5. The pumping device according to claim 1 wherein the microchannel is formed in a substrate and said electrodes are wires inserted through the substrate so that they protrude into said microchannel.

6. The pumping device according to claim 1 wherein said microchannel is formed in glass and said electrodes are wires inserted through holes in the glass, and wherein said holes are sealed with a conductive via fill ink.

7. The pumping device of claim 6, wherein the conductive via fill ink is sintered.

8. An array of parallel microchannels for conducting chemical reactions, for screening of biological organic and inorganic compounds, or for the assay of compounds, the array comprising parallel microchannels and a pumping device of claim 1 inserted in each microchannel.

9. The pumping device of claim 1, wherein the microchannel has a diameter of about 400 μ

m or less.

10. The pumping device of claim 1, wherein the microchannel has a diameter of from about 100 μ

m to about 400 μ

m.

11. The pumping device of claim 1, wherein the electrohydrodynamic pump comprises a first electrode and a second electrode from among the electrodes situated along the length of the microchannel and the electro-osmotic pump comprises a third electrode and a fourth electrode from among the electrodes situated along the length of the microchannel.