Molecular biological diagnostic systems including electrodes
First Claim
1. An electronic device adapted for performing molecular biological processes comprising:
- a support having a first generally planar surface,a plurality of microlocations, the microlocations including an electrode disposed on the first surface of the support and a permeation layer disposed above the electrode,individual electrical connections to the electrodes, andan optical access to the electrodes.
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Accused Products
Abstract
A system for performing molecular biological diagnosis, analysis and multi-step and multiplex reactions utilizes a self-addressable, self-assembling microelectronic system for actively carrying out controlled reactions in microscopic formats. These reactions include most molecular biological procedures, such as nucleic acid hybridization, antibody/antigen reaction, and clinical diagnostics. Multi-step combinatorial biopolymer synthesis may be performed. A controller interfaces with a user via input/output devices, preferably including a graphical display. Independent electronic control is achieved for the individual microlocations. In the preferred embodiment, the controller interfaces with a power supply and interface, the interface providing selective connection to the microlocations, polarity reversal, and optionally selective potential or current levels to individual electrodes. A system for performing sample preparation, hybridization and detection and data analysis integrates multiple steps within a combined system. Charged materials are transported preferably via free field electrophoresis. DNA complexity reduction is achieved preferably by binding of DNA to a support, followed by cleaving unbound materials, such as by restriction enzymes, followed by transport of the cleaved DNA fragments. Active, programmable matrix devices are formed in a variety of formats, including a square matrix pattern with fanned out electrical connections, an array having electrical connections and optionally optical connections from beneath the specific microlocations. A highly automated DNA diagnostic system results.
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Citations
60 Claims
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1. An electronic device adapted for performing molecular biological processes comprising:
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a support having a first generally planar surface, a plurality of microlocations, the microlocations including an electrode disposed on the first surface of the support and a permeation layer disposed above the electrode, individual electrical connections to the electrodes, and an optical access to the electrodes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. An electronic system composed of an integrated arrangement of component devices for performing biological sample processing and molecular biological analyses comprising:
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a support for the integrated component devices, the support adapted to receive sample containing material to be analyzed, an active sample preparation component device to which the sample material to be analyzed may be applied, the active sample preparation component device including means for electrophoretically transporting said material within the sample preparation component device, wherein the material is processed to generate relative purified analytes and the resultant analytes are transported to a subsequent integrated component device, and an active, programmable electronic analytical component device including a plurality of separately addressable microlocations. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55)
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56. An electronic device adapted for performing molecular biological processes comprising:
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a support having a first generally planar surface, a plurality of microlocations disposed on the first surface of the support, the microlocations including an electrode having a contacting portion adjacent the first surface of the support and a permeation layer disposed above the electrode, individual electrical connections to the electrodes, and an optical access to the microlocations. - View Dependent Claims (57, 58, 59, 60)
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Specification