Evanescent scanning of biochemical array

• US 5,633,724 A
• Filed: 08/29/1995
• Issued: 05/27/1997
• Est. Priority Date: 08/29/1995
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Hewlett-Packard Company (HP Inc.)
• Inventors: King, David A., Seher, Jens-Peter
• Primary Examiner(s): Gonzalez, Frank
• Assistant Examiner(s): MERLINO, AMANDA H
• Application Number:
  US08/520,456
• Time in Patent Office: 637 Days
• Field of Search: 356/445, 356/352, 356/136, 422/82.08, 422/82.11
• US Class Current: 356/445
• CPC Class Codes: G01N 21/552 Attenuated total reflection
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7746 the waveguide coupled to a cavity resonator

Abstract

An apparatus for detecting a target substance in a pixel array is provided. The apparatus has a light source to emit a light suitable for exciting the target substance in the array, a total internal reflection (TIR) member, and a light detector. The TIR member has a TIR surface on which the array is located. The light from the light source passes into the TIR member and is reflected by the TIR surface. The array is within an evanescent field region at the TIR surface. The light detector is adapted to detect light emitted from the array as a result of evanescent excitation of the target substance.

Claims

1. An apparatus for detecting a target substance in a pixel array comprising:

(a) a light source to emit a light suitable for exciting the target substance;

(b) a total internal reflection (TIR) member having a TIR surface on which a pixel array is located, the light from the light source passing into the TIR member and is reflected by the TIR surface, the pixel array being within an evanescent field region at the TIR surface; and

(c) a light detector for detection of light emitted from the pixel array as a result of evanescent excitation of the target substance.

2. The apparatus of claim 1 further comprising an optical resonator that has a resonance cavity for the light generated by the light source wherein the TIR member is located within the resonance cavity such that light traveling within the resonance cavity impinges on the TIR surface.

3. The apparatus of claim 2 wherein the light source is a gain medium of a laser and is located outside the resonance cavity.

4. The apparatus of claim 2 wherein the light source is an antireflection-coated semiconductor laser.

5. The apparatus of claim 1 wherein the light from the light source is reflected substantially without loss by the TIR surface.

6. The apparatus of claim 1 wherein the light source irradiates all the pixels of the pixel array simultaneously.

7. The apparatus of claim 1 wherein the TIR member is stationary during light detection.

8. The apparatus of claim 1 wherein the light detector is motionless during light detection.

9. The apparatus of claim 1 further comprising a prism and a refractive index match/ng film wherein the refractive index matching film is disposed between the TIR member and the prism such that the light from the light source passes through the prism, the refractive index matching layer and the TIR member before impinging on the TIR surface.

10. The apparatus of claim 1 wherein the light detector is a two dimensional array detector.

11. The apparatus of claim 1 wherein the apparatus further comprises an optical filter and optical fibers for transferring light from the optical filter to the detector.

12. The apparatus of claim 1 wherein pixels in the pixel array are pixels having polymeric sections for coupling with polymeric target substances.

13. A method of analyzing a pixel array of chemical molecules for a target substance, comprising:

(a) contacting a chemical array with a sample suspected of containing a target substance to form the pixel array;

(b) directing light from a light source to pass into a total internal reflection (TIR) member having a TIR surface and an array surface on which the pixel array is located to result in an evanescent field region such that the pixel array is within the evanescent field region at the TIR surface, thereby causing excitation of the target substance on the pixel array, the light from the light source being reflected from the TIR surface; and

(c) detecting light emitted from the pixel array as a result of the excitation of the target substance caused by the evanescent field.

14. The method of claim 13 further comprising mounting the TIR member inside an optical resonator that has a resonance cavity for the light generated by the light source such that light traveling within the resonance cavity impinges on the TIR surface.

15. The method of claim 13 wherein the light from the light source is reflected by the TIR surface substantially without loss.

16. The method of claim 13 wherein the light source irradiates all pixels of the pixel array simultaneously.

17. The method of claim 13 wherein the TIR member is stationary during light detection.

18. The method of claim 13 wherein the light detector is motionless during light detection.

19. A method of making an apparatus for detecting a target substance on a pixel array comprising:

(a) mounting a total internal reflection (TIR) member on a support, the TIR member having a TIR surface on which the pixel array can be located, the pixel array being capable of binding the target substance;

(b) mounting a light source on the support to irradiate a light suitable for exciting the target substance on a pixel array located on the TIR surface such that the light from the light source passes into the TIR member and is reflected by the TIR surface to result in an evanescent field region, the pixel array being within the evanescent field region at the TIR surface; and

(c) mounting a light detector on the support for detecting light emitted from the pixel array as a result of the evanescent excitation of the target substance.