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Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture

  • US 5,635,386 A
  • Filed: 11/02/1994
  • Issued: 06/03/1997
  • Est. Priority Date: 06/15/1989
  • Status: Expired due to Term
First Claim
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1. A method for controlling cellular lineage development in an ex vivo human hematopoietic cellular system, comprising culturing human stem and/or progenitor cells found in the human hematopoietic system in liquid culture media containing hematopoietic growth factors and which is replaced at a rate which is either (i) substantially continuous and providing ex vivo human stem cell division and/or human progenitor cell expansion therein or (ii) equal to 50 to 100% daily replacement for a cell density of from 1×

  • 104 to 1×

    107 cells per ml of culture, while maintaining said culture under physiologically acceptable conditions and adjusting the concentration of said hematopoietic growth factors as follows;

    IL-3 or GM-CSF at a concentration of 0.1 to 100 ng/ml/day, Epo at a concentration of 0.001 to 10 U/ml/day, steel factor at a concentration of 1-100 ng/ml/day, IL-1 at a concentration of 10-100 U/ml/3-5 days, IL-6, G-CSF, bFGF, IL-7, IL-8, IL-9, IL-10, IL-11, PDGF or EGF at a concentration of 1-100 ng/ml/day, and mixtures thereof, to select for enhanced production of a desired human hematopgietic cell type.

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