Method of manufacturing a cell culture apparatus from inorganic materials
First Claim
1. A method for making a cell culture apparatus comprising the steps of:
- providing a substrate; and
depositing an inorganic film having a thickness of less than about 0.5 μ
m on the substrate to have a relatively smooth surface with a predetermined surface roughness defining surface indentations smaller than a size of corresponding cells to be grown thereon so that cells grown thereon adhere by chemical adhesion rather than by mechanical interlocking.
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Abstract
A cell culture apparatus includes a substrate, and an inorganic film on the substrate and having a relatively smooth surface for cell growth thereon. Moreover, the relatively smooth surface has a relatively low predetermined surface roughness so that cells grown thereon adhere by chemical adhesion rather than by mechanical interlocking. In other words, the predetermined surface roughness defines surface indentations smaller than a size of corresponding cells to be grown thereon. Accordingly, the interaction between various inorganic materials and cells may be studied based upon chemical adhesion rather than mechanical interlocking. The predetermined surface roughness is preferably less about 10 μm root-mean-square, and more preferably less than about 1 μm root-mean-square. The relatively smooth growth surface is preferably obtained by thermal vacuum evaporation and deposition, followed by annealing of the as-deposited film.
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Citations
16 Claims
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1. A method for making a cell culture apparatus comprising the steps of:
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providing a substrate; and depositing an inorganic film having a thickness of less than about 0.5 μ
m on the substrate to have a relatively smooth surface with a predetermined surface roughness defining surface indentations smaller than a size of corresponding cells to be grown thereon so that cells grown thereon adhere by chemical adhesion rather than by mechanical interlocking. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for making a cell culture apparatus comprising the steps of:
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providing a substrate; and depositing an inorganic film on said substrate, said inorganic film having a smooth surface for promoting cell growth thereon by chemical adhesion, the smooth surface having a surface roughness less than about 10 μ
m root-mean-square defining surface indentations smaller than a size of corresponding cells to be grown thereon so that cells grown thereon adhere by chemical adhesion rather than by mechanical interlocking. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
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Specification
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- Resources
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Current AssigneeCharlotte Mecklenburg Hospital Authority
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Original AssigneeCharlotte Mecklenburg Hospital Authority
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InventorsPeindl, Richard D., Ramp, Warren K.
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Primary Examiner(s)CANO, MILTON I
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Application NumberUS08/642,122Time in Patent Office412 DaysField of Search435/240.1, 435/240.23, 435/240.243, 435/284.1, 435/369.1, 356/396, 356/398, 623/1, 623/66, 427/2.24, 427/2.25, 427/491US Class Current435/402CPC Class CodesC12M 23/10 Petri dish crystallising di...C12M 23/20 Material Coatings immunocoa...C12M 25/02 Membranes; Filters filters ...
