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Ligands of HIV-1 TAT protein

  • US 5,637,461 A
  • Filed: 03/24/1995
  • Issued: 06/10/1997
  • Est. Priority Date: 06/11/1990
  • Status: Expired due to Term
First Claim
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1. A method for identifying non-naturally occurring single-stranded nucleic acid ligands to the HIV-1 tat protein comprising:

  • a) preparing a candidate mixture comprising non-naturally occurring single-stranded nucleic acids;

    b) contacting said candidate mixture with the HIV-1 tat protein, wherein non-naturally occurring single-stranded nucleic acids having an increased affinity to the HIV-1 tat protein relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;

    c) partitioning said increased affinity non-naturally occurring single-stranded nucleic acids from the remainder of the candidate mixture; and

    d) amplifying said increased affinity non-naturally occurring single-stranded nucleic acids to yield a mixture of non-naturally occurring single-stranded nucleic acids enriched in non-naturally occurring single-stranded nucleic acids having increased affinity to the HIV-1 tat protein, whereby non-naturally occurring single-stranded nucleic acid ligands to the HIV-1 tat protein are identified.

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