Ligands of HIV-1 TAT protein
First Claim
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1. A method for identifying non-naturally occurring single-stranded nucleic acid ligands to the HIV-1 tat protein comprising:
- a) preparing a candidate mixture comprising non-naturally occurring single-stranded nucleic acids;
b) contacting said candidate mixture with the HIV-1 tat protein, wherein non-naturally occurring single-stranded nucleic acids having an increased affinity to the HIV-1 tat protein relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
c) partitioning said increased affinity non-naturally occurring single-stranded nucleic acids from the remainder of the candidate mixture; and
d) amplifying said increased affinity non-naturally occurring single-stranded nucleic acids to yield a mixture of non-naturally occurring single-stranded nucleic acids enriched in non-naturally occurring single-stranded nucleic acids having increased affinity to the HIV-1 tat protein, whereby non-naturally occurring single-stranded nucleic acid ligands to the HIV-1 tat protein are identified.
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Abstract
Methods are described for the identification of nucleic acid ligand solutions to the HIV-1 tat protein. Motifs I, II and III are nucleic acid ligands identified by the disclosed methods.
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Citations
3 Claims
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1. A method for identifying non-naturally occurring single-stranded nucleic acid ligands to the HIV-1 tat protein comprising:
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a) preparing a candidate mixture comprising non-naturally occurring single-stranded nucleic acids; b) contacting said candidate mixture with the HIV-1 tat protein, wherein non-naturally occurring single-stranded nucleic acids having an increased affinity to the HIV-1 tat protein relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; c) partitioning said increased affinity non-naturally occurring single-stranded nucleic acids from the remainder of the candidate mixture; and d) amplifying said increased affinity non-naturally occurring single-stranded nucleic acids to yield a mixture of non-naturally occurring single-stranded nucleic acids enriched in non-naturally occurring single-stranded nucleic acids having increased affinity to the HIV-1 tat protein, whereby non-naturally occurring single-stranded nucleic acid ligands to the HIV-1 tat protein are identified. - View Dependent Claims (2, 3)
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Specification