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Modulated homogeneous fluorescence biospecific affinity assay employing fluorescing lanthanide chelates as covalent labels

  • US 5,637,509 A
  • Filed: 12/12/1990
  • Issued: 06/10/1997
  • Est. Priority Date: 09/24/1987
  • Status: Expired due to Term
First Claim
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1. In the method for a homogenous biospecific affinity assay for determining the content of an analyte in a biological sample, said assay being carried out in an aqueous reaction medium by means of a biospecific affinity reactant labelled with a lanthanide chelate in which a lanthanide ion capable of exhibiting ionic fluorescence is chelated by a ligand bound covalently to the reactant, and said fluorescence being measured by means of time-resolved fluorescence spectroscopy, the improvement comprising that(i) the lanthanide chelate formed by the lanthanide ion and the covalently bound ligand exhibits fluorescence, and that(ii) a fluorescence modulator is added to the reaction medium in an amount so that the lanthanide fluorescence as measured from the medium becomes correlated to the analyte concentration therein, and effects on the lanthanide fluorescence from sample constituents other than analyte become negligible.

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