High-affinity oligonucleotide ligands to the tachykinin substance P
First Claim
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1. A nucleic acid ligand to Substance P (SP) identified according to a process comprising the steps of:
- a) contacting a candidate mixture of nucleic acids with SP, wherein nucleic acids having an increased affinity to the SP relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and
c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched in nucleic acids having increased affinity to SP, whereby a nucleic acid ligand of SP may be identified.
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Abstract
Methods are described for the identification and preparation of nucleic acid ligands to Substance P (SP). Included in the invention are specific RNA ligands to SP identified by the SELEX method.
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7 Claims
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1. A nucleic acid ligand to Substance P (SP) identified according to a process comprising the steps of:
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a) contacting a candidate mixture of nucleic acids with SP, wherein nucleic acids having an increased affinity to the SP relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched in nucleic acids having increased affinity to SP, whereby a nucleic acid ligand of SP may be identified. - View Dependent Claims (2, 3, 4, 7)
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- 5. A purified and isolated non-naturally occurring nucleic acid ligand to SP.
Specification