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Luminescent lanthanide chelates and methods of use

  • US 5,639,615 A
  • Filed: 12/09/1996
  • Issued: 06/17/1997
  • Est. Priority Date: 06/29/1994
  • Status: Expired due to Term
First Claim
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1. A method of detecting a portion of a sample by luminescence, said method comprising the steps of:

  • contacting a sample portion with a luminescent complex of a lanthanide chelate and a lanthanide capable of binding said chelate with an equilibrium constant of at least 109 M-1 ;

    said lanthanide chelate comprising a lanthanide chelator covalently joined to a sensitizer. wherein a complex of said chelate and said lanthanide is capable of enhanced lanthanide luminescence, and said sensitizer comprises a polynuclear heterocyclic aromatic compound of the general formula;

    ##STR6## wherein X comprises an atom from periodic group 5 or 6, wherein a single position 2-8 carbon atom of said sensitizer is substituted with a linking up through which said sensitizer is covalently joined to said chelator;

    exposing said sample portion to light at a first wavelength capable of inducing a first electronic transition in said chelate;

    detecting a localized emission of light from said sample at a second wavelength, wherein said second wavelength is longer than said first wavelength and results from a second electronic transition in said chelate;

    wherein the localized detection of said emission of light correlates with the localized presence of said sample portion.

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