Electrochemical determination of fructosamine
First Claim
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1. A method for electrochemically determining the concentration of a fructosamine in a body fluid sample of a patient comprising the steps of:
- a. contacting said body fluid sample with a buffering agent and a biosensor, said biosensor comprising an anode and a cathode;
b. applying a voltage to the anode;
c. measuring the electrical current passing between the anode and cathode; and
d. comparing the amount of current measured to a standard curve of current versus concentration of fructosamine, as determined for said body fluid in order to determine the concentration of the fructosamine in said body fluid;
wherein said buffering agent adjusts the pH of body fluid sample to between about 10 to about 12.5, and said buffering agent may be on the biosensor or otherwise added to the sample before the current is measured.
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Abstract
The present invention relates to a method for electrochemically measuring the concentration of fructosamine, or its high alkalinity eneaminol tautomer in a body fluid sample.
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Citations
28 Claims
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1. A method for electrochemically determining the concentration of a fructosamine in a body fluid sample of a patient comprising the steps of:
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a. contacting said body fluid sample with a buffering agent and a biosensor, said biosensor comprising an anode and a cathode; b. applying a voltage to the anode; c. measuring the electrical current passing between the anode and cathode; and d. comparing the amount of current measured to a standard curve of current versus concentration of fructosamine, as determined for said body fluid in order to determine the concentration of the fructosamine in said body fluid; wherein said buffering agent adjusts the pH of body fluid sample to between about 10 to about 12.5, and said buffering agent may be on the biosensor or otherwise added to the sample before the current is measured. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for electrochemically determining the concentration of a fructosamine in a body fluid sample of a patient, comprising the steps of:
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a. contacting the body fluid sample with one or more mediators, a buffering agent and a biosensor comprised of an anode and cathode; b. applying a voltage to the anode; c. measuring the electrical current passing between the anode and cathode; and d. comparing the amount of current measured to a standard curve of current versus concentration of fructosamine in the presence of said one or more mediators, as determined for said body fluid; wherein said buffering agent and mediator are on the biosensor or otherwise added to the sample before said current is measured; and
said buffering agent adjusts the pH of said body fluid sample to between about 4 to about 12.5. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method for electrochemically determining the concentration of a fructosamine in a body fluid sample of a patient, comprising the steps of:
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a. contacting the body fluid sample with an oxidoreductase enzyme, a buffering agent and a biosensor comprised of an anode and cathode; b. applying a voltage to the anode; c. measuring the electrical current passing between the anode and cathode; and d. comparing the amount of current measured to a standard curve of current versus concentration of fructosamine in the presence of said oxidoreductase enzyme, as determined for said body fluid; wherein said buffering agent and enzyme are on the biosensor or otherwise added to the sample before said current is measured; and
said buffering agent adjusts the pH of said body fluid sample to between about 4 to about 10. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28)
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Specification