High-affinity RNA ligands for basic fibroblast growth factor
First Claim
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1. A nucleic acid ligand to basic fibroblast growth factor (bFGF) identified according to the method comprising:
- a) preparing a candidate mixture of nucleic acids;
b) contacting the candidate mixture with bFGF, wherein nucleic acids having an increased affinity to bFGF relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
c) partitioning the increased affinity nucleic acid ligands from the remainder of the candidate mixture; and
d) amplifying the increased affinity nucleic acid ligands for bFGF to yield a mixture of nucleic acids enriched for sequences with a higher affinity to bFGF, whereby a nucleic acid ligand of bFGF may be identified.
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Abstract
Methods are described for the identification and preparation of nucleic acid ligand solutions to basic fibroblast growth factor (bFGF). Included in the invention are nucleic acid ligands to bFGF which are inhibitors of bFGF and 2'"'"'-amino-modified RNA ligands to bFGF.
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9 Claims
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1. A nucleic acid ligand to basic fibroblast growth factor (bFGF) identified according to the method comprising:
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a) preparing a candidate mixture of nucleic acids; b) contacting the candidate mixture with bFGF, wherein nucleic acids having an increased affinity to bFGF relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; c) partitioning the increased affinity nucleic acid ligands from the remainder of the candidate mixture; and d) amplifying the increased affinity nucleic acid ligands for bFGF to yield a mixture of nucleic acids enriched for sequences with a higher affinity to bFGF, whereby a nucleic acid ligand of bFGF may be identified. - View Dependent Claims (2, 3, 4, 9)
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- 5. A purified and isolated non-naturally occurring nucleic acid ligand to bFGF.
Specification