Fiber-optic detectors with terpolymeric analyte-permeable matrix coating

• US 5,640,470 A
• Filed: 03/27/1995
• Issued: 06/17/1997
• Est. Priority Date: 03/27/1995
• Status: Expired due to Term

• Current Assignee: ICU Medical Incorporated, Hospira Incorporated (Pfizer Inc.)
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Iyer, Lokanathan M., Zhao, Yanqun
• Primary Examiner(s): Bovernick, Rodney B.
• Assistant Examiner(s): Sanghavi, Hemang
• Application Number:
  US08/410,810
• Time in Patent Office: 813 Days
• Field of Search: 385/12, 385/116, 385/129, 385/130, 422/82.06, 422/82.07, 128/634
• US Class Current: 385/12
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G02B 6/241 Light guide terminations

Abstract

The invention provides fiber-optic detectors with rapid response times for measuring analytes in aqueous solutions, including blood. The detectors, because of their size, accuracy, and sterilizability may be used in vitro. In a preferred embodiment, pellets comprising a unique, permeable terpolymer of methyl methacrylate, butyl acrylate and methacrylamidopropyltrimethylammonium chloride; and an indicator for an analyte are used as the detecting element of a fiber-optic sensor. Response times for pH testing are better than 150 seconds at t90%. The pellets are easily made by solvent casting techniques and failure rates are low.

Claims

1. A fiber-optic blood analyte detector comprising:

at least one fiber-optic filament with an end having a detecting element for immersion in blood to be analyzed, the detecting element comprising an analyte-permeable matrix for detecting analytes in said blood, the matrix comprising;

(i) a terpolymer of methyl methacrylate, butyl acrylate and methacrylamidopropyltrimethylammonium chloride; and

(ii) an indicator for analytes to be detected in blood;

wherein said matrix has a permeability to the analytes that allows detection of pH at t90% in less than about 150 seconds.

2. The detector of claim 1, wherein the matrix is a terpolymer comprising from about 85 to about 60 mole percent methyl methacrylate;

from about 10 to about 25 mole percent of butyl acrylate; and

from about 5 to about 10 mole percent of methacrylamidopropyltrimethylammonium chloride.

3. The detector of claim 1, wherein the matrix is in a pelletized form and is adhesively bonded to the end of at the least one fiber-optic filament;

and the indicator is chemically bonded to the matrix.

4. The detector of claim 3, wherein the indicator is selected from the group consisting of phenol red, methylene blue, cresol red, chlorophenol red, carboxynaphtholthaleine, and α

-chlorocresol.

5. The detector of claim 3, wherein the detecting element further comprises a metallic reflector.

6. The detector of claim 5, further comprising a protective semipermeable membrane over the matrix.

7. The detector of claim 1, wherein the matrix is in the form of a membrane adhesively attached to at least the end of the at least one filament.

8. The detector of claim 7, wherein the indicator is in the form of particles, said particles enclosed by the membrane so that light emitting from one end of a fiber-optic filament impinges on said particles, is reflected from said particles, and is then detected.

9. The detector of claim 8, wherein the indicator is selected from the group consisting of cresol red, chlorophenol red, carboxynaphtholthaleine, and α

-chlorocresol.

10. A fiber-optic blood analyte detector comprising:

at least one fiber-optic filament with an end having a detecting element for immersion in blood to be analyzed;

the detecting element comprising an analyte-permeable matrix for detecting analytes in said blood, the matrix comprising a terpolymer comprising from about 85 to about 60 mole percent methyl methacrylate, from about 10 to about 25 mole percent butyl acrylate, and from about 5 to about 10 mole percent of methacrylarnidopropyltrimethylammonium chloride; and

an indicator for analytes to be detected in blood.

11. The detector of claim 10, wherein the matrix has a thickness ranging from about 15 to 50 microns.

12. The detector of claim 10, wherein the matrix comprises an indicator selected from the group consisting of phenol red, methylene blue, cresol red, chlorophenol red, carboxynaphtholthaleine, and α

-chlorocresol chemically bonded to the terpolymer.

13. The detector of claim 12, wherein the detecting element further comprises a metallic reflector.

14. The detector of claim 13, further comprising a protective semipermeable membrane over the matrix.

15. The detector of claim 10, wherein the matrix is in a pellet form and is adhesively bonded to the end of at the least one fiber-optic filament.

16. The detector of claim 15, wherein the detecting element further comprises a metallic reflector;

and the matrix is covered with a protective semipermeable coating.

17. The detector of claim 10, wherein the matrix is a thin membrane enclosing a particulate indicator, said membrane adhesively bonded to the end of the at least one fiber-optic filament.

18. A fiber-optic detector for detecting changes in the pH of blood, comprising:

at least one fiber-optic filament with a detecting element at one end thereof for immersion in blood to be analyzed for pH;

the detecting element comprising;

(i) an analyte-permeable matrix comprising a terpolymer of from about 85 to about 60 mole percent methyl methacrylate, from about 10 to about 25 mole percent butyl acrylate, and from about 5 to about 10 mole percent methacrylamidopropyltrimethylammonium chloride; and

(ii) an indicator sensitive to and changing light reflected therefrom in response to changes in hydrogen ion concentration, wherein said matrix has a permeability to hydrogen ions that allows detection of pH at t90% in less than about 150 seconds.

19. The detector of claim 18, wherein the matrix is in the form of a pellet adhered to the one end of at the least one fiber-optic filament, and the indicator is chemically bonded to the terpolymer of the matrix.

20. The detector of claim 18, wherein the matrix is in the form of a membrane surrounding the indicator, in particulate form, the membrane adhesively attached to the one end of the at least one fiber-optic filament.