Non-invasive method for isolation and detection of fetal DNA
First Claim
1. A noninvasive method for facilitating prenatal diagnosis of a chromosomal abnormality in a fetus, comprising:
- obtaining a peripheral blood sample from a pregnant woman;
treating the peripheral blood sample to produce an enriched blood sample enriched in fetal nucleated erythrocytes; and
performing in situ hybridization on the enriched blood sample with a chromosome specific probe or a probe specific for abnormal chromosomal DNA to generate spatially resolved signals in individual chromosomes of interphase cells such that diagnosis of a chromosomal abnormality in the fetus is facilitated on the basis of the generated signals.
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Abstract
A method of detecting the presence or absence of a fetal DNA sequence of interest in fetal DNA derived from a sample of peripheral blood obtained from a pregnant woman is described. The method involves obtaining a sample of peripheral blood from a pregnant woman, treating the sample of peripheral blood such that the fetal DNA present in the fetal nucleated cells is made available for detection and detecting the presence or absence of the fetal DNA sequence of interest in the available fetal DNA. The proportion of fetal nucleated cells present in the sample of peripheral blood can be increased forming a sample enriched in fetal nucleated cells prior to the detection step. The fetal DNA sequence of interest can be detected by treating the peripheral blood sample such that fetal DNA present in the sample is made available for hybridization with a DNA probe and subsequently contacting the available fetal DNA with a DNA probe hybridizable to fetal DNA of interest under hybridization conditions. The presence or absence of hybridization between the DNA probe and the fetal DNA of interest is detected as an indication of the presence or absence of the fetal DNA of interest.
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Citations
26 Claims
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1. A noninvasive method for facilitating prenatal diagnosis of a chromosomal abnormality in a fetus, comprising:
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obtaining a peripheral blood sample from a pregnant woman; treating the peripheral blood sample to produce an enriched blood sample enriched in fetal nucleated erythrocytes; and performing in situ hybridization on the enriched blood sample with a chromosome specific probe or a probe specific for abnormal chromosomal DNA to generate spatially resolved signals in individual chromosomes of interphase cells such that diagnosis of a chromosomal abnormality in the fetus is facilitated on the basis of the generated signals. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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Specification