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Method of assaying for an analyte using surface plasmon resonance

  • US 5,641,640 A
  • Filed: 12/29/1994
  • Issued: 06/24/1997
  • Est. Priority Date: 06/29/1992
  • Status: Expired due to Fees
First Claim
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1. A method for assaying for an analyte in a fluid sample comprising,measuring a change in refractive index at a solid optical surface in contact with the sample, wherein said change is caused by the analyte affecting the binding or release of a refractive index changing species to or from the optical surface, wherein the refractive index of said refractive index enhancing species varies with wavelength;

  • determining the variation with wavelength of said change of refractive index caused by the variation of refractive index with wavelength of said refractive index enhancing species, for a number of discrete wavelengths or for a continuous range of wavelengths; and

    correlating said variation to the amount of analyte in said fluid sample.

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