Method of assaying for an analyte using surface plasmon resonance
First Claim
1. A method for assaying for an analyte in a fluid sample comprising,measuring a change in refractive index at a solid optical surface in contact with the sample, wherein said change is caused by the analyte affecting the binding or release of a refractive index changing species to or from the optical surface, wherein the refractive index of said refractive index enhancing species varies with wavelength;
- determining the variation with wavelength of said change of refractive index caused by the variation of refractive index with wavelength of said refractive index enhancing species, for a number of discrete wavelengths or for a continuous range of wavelengths; and
correlating said variation to the amount of analyte in said fluid sample.
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Abstract
In a method of assaying for an analyte in a fluid sample, the presence of the analyte is detected by determining the resulting change in refractive index at a solid optical surface in contact with the sample, which change is caused by the analyte involving or influencing the binding or release of a refractive index enhancing species to or from, respectively, the optical surface, the refractive index of the refractive index enhancing species varying with wavelength. According to the invention, the determination comprises determining the variation with wavelength of the resulting change of refractive index, caused by the variation of refractive index with wavelength of the refractive index enhancing species, for a number of discrete wavelengths or for a continuous range of wavelengths. This variation is representative of the amount of analyte.
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Citations
14 Claims
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1. A method for assaying for an analyte in a fluid sample comprising,
measuring a change in refractive index at a solid optical surface in contact with the sample, wherein said change is caused by the analyte affecting the binding or release of a refractive index changing species to or from the optical surface, wherein the refractive index of said refractive index enhancing species varies with wavelength; -
determining the variation with wavelength of said change of refractive index caused by the variation of refractive index with wavelength of said refractive index enhancing species, for a number of discrete wavelengths or for a continuous range of wavelengths; and correlating said variation to the amount of analyte in said fluid sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification