Human papillomavirus type 52 DNA sequences and methods for employing the same
DCFirst Claim
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1. A recombinant DNA of HPV 52 comprising a cloning vector and HPV 52 DNA,wherein the length of the HPV 52 DNA is between approximately 15 and 8000 nucleotide bases,wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, andwherein the HPV 52 DNA does not hybridize to DNA from HPV types 1 through 51 under stringent conditions.
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Abstract
Nucleic acid hybridization probes for human papillomavirus types and particularly human papillomavirus type 52; and methods for employing the same.
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Citations
27 Claims
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1. A recombinant DNA of HPV 52 comprising a cloning vector and HPV 52 DNA,
wherein the length of the HPV 52 DNA is between approximately 15 and 8000 nucleotide bases, wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and wherein the HPV 52 DNA does not hybridize to DNA from HPV types 1 through 51 under stringent conditions.
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8. Essentially pure HPV 52 DNA, wherein the length of the HPV 52 DNA is between approximately 15 and 8000 nucleotide bases,
wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and wherein the HPV 52 DNA does not hybridize to DNA from HPV types 1 through 51 under stringent conditions.
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13. Essentially pure HPV 52 RNA, wherein the length of the HPV 52 RNA is between approximately 15 and 8000 nucleotide bases,
wherein the HPV 52 RNA consists of all or a fragment of an HPV RNA, wherein the HPV RNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and wherein the HPV 52 RNA does not hybridize to DNA from HPV types 1 through 51 under stringent conditions.
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18. An HPV 52 hybridization probe comprising a member selected from the group consisting of
(i) HPV 52 DNA labelled with a detectable label, and (ii) HPV 52 RNA labelled with a detectable label, wherein the length of the HPV 52 DNA or HPV 52 RNA is between approximately 15 and 8000 nucleotide bases, wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, wherein the HPV 52 RNA consists of all or a fragment of an HPV RNA, wherein the HPV RNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and wherein the HPV 52 DNA and HPV 52 RNA do not hybridize to DNA from HPV types 1 through 51 under stringent conditions.
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21. An HPV hybridization probe composition comprising
(a) a member selected from the group consisting of (i) HPV 52 DNA labelled with a detectable label and (ii) HPV 52 RNA labelled with a detectable label, wherein the length of the HPV 52 DNA or HPV 52 RNA is between approximately 15 and 8000 nucleotide bases, wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, wherein the HPV 52 RNA consists of all or a fragment of an HPV RNA, wherein the HPV RNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and wherein the HPV 52 DNA and HPV 52 RNA do not hybridize to DNA from HPV types 1 through 51 under stringent conditions, and (b) DNA or RNA of at least one other HPV type labelled with a detectable label.
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24. A method for detecting HPV DNA or RNA comprising:
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(1) carrying out hybridization, under non-stringent conditions, with (a) a member selected from the group consisting of (i) HPV 52 DNA labelled with a detectable label, and (ii) HPV 52 RNA labelled with a detectable label, wherein the length of the HPV 52 DNA or HPV 52 RNA is between approximately 15 and 8000 nucleotide bases, wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, wherein the HPV 52 RNA consists of all or a fragment of an HPV RNA, wherein the HPV RNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and wherein the HPV 52 DNA and HPV 52 RNA do not hybridize to DNA from HPV types 1 through 51 under stringent conditions, and; (b) an unknown sample of DNA or RNA, and (2) assaying for the presence of hybridization so as to detect HPV DNA or RNA in said sample. - View Dependent Claims (25)
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26. A method for detecting HPV 52 DNA or RNA comprising:
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(1) carrying out hybridization, under stringent conditions, with (a) a member selected from the group consisting of (i) HPV 52 DNA labelled with a detectable label, and (ii) HPV 52 RNA labelled with a detectable label, wherein the length of the HPV 52 DNA or HPV 52 RNA is between approximately 15 and 8000 nucleotide bases, wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, wherein the HPV 52 RNA consists of all or a fragment of an HPV RNA that cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and wherein the HPV 52 DNA and HPV 52 RNA do not hybridize to DNA from HPV types 1 through 51 under stringent conditions, and; (b) an unknown sample of DNA or RNA, and (2) assaying for the presence of hybridization so as to detect HPV 52 DNA or RNA in said sample. - View Dependent Claims (27)
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Specification