Human papillomavirus type 52 DNA sequences and methods for employing the same

• US 5,643,715 A
• Filed: 03/23/1994
• Issued: 07/01/1997
• Est. Priority Date: 10/26/1988
• Status: Expired due to Term

• Current Assignee: Carla D. Demaria
• Original Assignee: Georgetown University
• Inventors: Lancaster, Wayne D.
• Primary Examiner(s): MOSHER, MARY
• Application Number:
  US08/217,015
• Time in Patent Office: 1,196 Days
• Field of Search: 435/235.1, 435/5, 435/6, 536/23.1, 536/23.72, 536/24.32, 935/78
• US Class Current: 435/5
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2267/02 Animal zootechnically ameliorated
• CPC Class Codes: A01K 2267/0337 Animal models for infectious diseases
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic
• CPC Class Codes: C12N 2710/20022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12Q 1/708 for papilloma

Abstract

Nucleic acid hybridization probes for human papillomavirus types and particularly human papillomavirus type 52; and methods for employing the same.

Claims

1. A recombinant DNA of HPV 52 comprising a cloning vector and HPV 52 DNA,

wherein the length of the HPV 52 DNA is between approximately 15 and 8000 nucleotide bases,

wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and

wherein the HPV 52 DNA does not hybridize to DNA from HPV types 1 through 51 under stringent conditions.

2. The recombinant DNA of HPV 52 as claimed in claim 1, wherein said cloning vector is selected from the group consisting of pBR322, pUCll, λ

charon, λ

L47, M13 derived bacteriophage, pZIP-Neo SV [Xl], pBKTK-1, pT712 and pT713.

3. The recombinant DNA of HPV 52 as claimed in claim 1, wherein said recombinant DNA of HPV 52 contains a non-protein encoding region of HPV 52 DNA, from the region between regions L1 and E6.

4. The recombinant DNA of HPV 52 of claim 1, wherein said recombinant DNA is labelled with a detectable label.

5. The recombinant HPV 52 DNA of claim 1 wherein the HPV 52 DNA is about 300 to 800 nucleotide bases in length.

6. The recombinant DNA of claim 5, wherein the HPV 52 DNA is about 300 to 800 nucleotide bases of the HPV portion of clone pCD15.

7. The recombinant DNA of HPV 52 as claimed in claim 1, wherein the HPV DNA is the HPV portion of clone pCD15.

8. Essentially pure HPV 52 DNA, wherein the length of the HPV 52 DNA is between approximately 15 and 8000 nucleotide bases,

wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and

wherein the HPV 52 DNA does not hybridize to DNA from HPV types 1 through 51 under stringent conditions.

9. The essentially pure HPV 52 DNA of claim 8, wherein said DNA is about 300 to about 800 nucleotide bases in length.

10. The essentially pure HPV 52 DNA as claimed in claim 9, wherein said HPV 52 DNA is derived from the non-protein encoding region of HPV 52 DNA between regions L1 and E6.

11. The essentially pure HPV 52 DNA of claim 8, wherein said HPV 52 DNA is labelled with a detectable label.

12. The essentially pure HPV 52 DNA as claimed in claim 8, wherein the HPV DNA is the HPV portion of clone pCD15.

13. Essentially pure HPV 52 RNA, wherein the length of the HPV 52 RNA is between approximately 15 and 8000 nucleotide bases,

wherein the HPV 52 RNA consists of all or a fragment of an HPV RNA, wherein the HPV RNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and

wherein the HPV 52 RNA does not hybridize to DNA from HPV types 1 through 51 under stringent conditions.

14. The essentially pure HPV 52 RNA of claim 13, wherein said RNA is about 300 to about 800 nucleotide bases in length.

15. The essentially pure HPV 52 RNA as claimed in claim 13, wherein the sequence of said HPV 52 RNA is the sequence or complement of the sequence of a non-protein encoding region of HPV 52 RNA from between regions L1 and E6.

16. The essentially pure HPV 52 RNA of claim 13, wherein said HPV 52 RNA is labelled with a detectable label.

17. The essentially pure HPV 52 RNA as claimed in claim 13, wherein the HPV RNA corresponds to the HPV portion of clone pCD15.

18. An HPV 52 hybridization probe comprising a member selected from the group consisting of

(i) HPV 52 DNA labelled with a detectable label, and

(ii) HPV 52 RNA labelled with a detectable label,

wherein the length of the HPV 52 DNA or HPV 52 RNA is between approximately 15 and 8000 nucleotide bases,

wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions,

wherein the HPV 52 RNA consists of all or a fragment of an HPV RNA, wherein the HPV RNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and

wherein the HPV 52 DNA and HPV 52 RNA do not hybridize to DNA from HPV types 1 through 51 under stringent conditions.

19. The HPV hybridization probe as claimed in claim 18, wherein said hybridization probe contains the sequence of the HPV 52 non-protein encoding region between regions L1 and E6.

20. The HPV 52 hybridization probe as claimed in claim 18, wherein the HPV DNA is the HPV portion of clone pCD15 and the HPV RNA corresponds to the HPV portion of clone pCD15.

21. An HPV hybridization probe composition comprising

(a) a member selected from the group consisting of

(i) HPV 52 DNA labelled with a detectable label and

(ii) HPV 52 RNA labelled with a detectable label,

wherein the length of the HPV 52 DNA or HPV 52 RNA is between approximately 15 and 8000 nucleotide bases,

wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions,

wherein the HPV 52 RNA consists of all or a fragment of an HPV RNA, wherein the HPV RNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and

wherein the HPV 52 DNA and HPV 52 RNA do not hybridize to DNA from HPV types 1 through 51 under stringent conditions, and

(b) DNA or RNA of at least one other HPV type labelled with a detectable label.

22. The HPV hybridization probe composition as claimed in claim 21, wherein said HPV hybridization probe composition contains the sequence of the HPV non-protein encoding region between regions L1 and E6.

23. The HPV 52 hybridization probe composition as claimed in claim 21, wherein the HPV DNA is the HPV portion of clone pCD15 and the HPV RNA corresponds to the HPV portion of clone pCD15.

24. A method for detecting HPV DNA or RNA comprising:

(1) carrying out hybridization, under non-stringent conditions, with

(a) a member selected from the group consisting of

(i) HPV 52 DNA labelled with a detectable label, and

(ii) HPV 52 RNA labelled with a detectable label,

wherein the length of the HPV 52 DNA or HPV 52 RNA is between approximately 15 and 8000 nucleotide bases,

wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions,

wherein the HPV 52 RNA consists of all or a fragment of an HPV RNA, wherein the HPV RNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and

wherein the HPV 52 DNA and HPV 52 RNA do not hybridize to DNA from HPV types 1 through 51 under stringent conditions, and;

(b) an unknown sample of DNA or RNA, and

(2) assaying for the presence of hybridization so as to detect HPV DNA or RNA in said sample.

25. The method of claim 24, wherein the HPV DNA is the HPV portion of clone pCD15 and the HPV RNA corresponds to the HPV portion of clone pCD15.

26. A method for detecting HPV 52 DNA or RNA comprising:

(1) carrying out hybridization, under stringent conditions, with

(a) a member selected from the group consisting of

(i) HPV 52 DNA labelled with a detectable label, and

(ii) HPV 52 RNA labelled with a detectable label,

wherein the length of the HPV 52 DNA or HPV 52 RNA is between approximately 15 and 8000 nucleotide bases,

wherein the HPV 52 DNA consists of all or a fragment of an HPV DNA, wherein the HPV DNA cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions,

wherein the HPV 52 RNA consists of all or a fragment of an HPV RNA that cross-hybridizes to the HPV portion of clone pCD15 to greater than 50% under moderately stringent conditions, and

wherein the HPV 52 DNA and HPV 52 RNA do not hybridize to DNA from HPV types 1 through 51 under stringent conditions, and;

(b) an unknown sample of DNA or RNA, and

(2) assaying for the presence of hybridization so as to detect HPV 52 DNA or RNA in said sample.

27. The method of claim 26, wherein the HPV DNA is the HPV portion of clone pCD15 and the HPV RNA corresponds to the HPV portion of clone pCD15.