Bioreagent immobilization medium

• US 5,643,721 A
• Filed: 02/09/1994
• Issued: 07/01/1997
• Est. Priority Date: 02/09/1994
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Schultz, Steven G., Vogdes, Sheila A., Brackett, John M., Spring, Thomas G.
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US08/193,972
• Time in Patent Office: 1,238 Days
• Field of Search: 252/500, 252/502, 252/503, 252/504, 252/506, 252/507, 252/508, 252/510, 252/511, 252/514, 252/516, 252/518, 252/520, 204/400, 204/703, 204/409, 204/290 R, 435/174, 435/177, 435/176, 435/178, 435/179, 435/180, 435/817, 435/25, 435/6, 436/518, 436/524, 436/525, 436/527, 436/528, 436/529, 436/531, 436/533, 436/534, 436/806, 436/535, 422/82.01
• US Class Current: 435/6.16
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/5436 with ligand physically entrapped within the solid phase liposomes G01N33/5432; immunological test elements G01N33/54386
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

The present invention provides an immobilization medium which can immobilize bioreagents to support materials and which dries to a water resistant layer or film. The immobilization medium comprises (i) a liquid or fluid binding reagent and (ii) complexes of a bioreagent immobilized to a solid phase which are evenly dispersed within the binding reagent. The suspension can further include supplemental ingredients evenly dispersed throughout the medium which can provide the medium with electrochemical properties, enhance the stability of the immobilized bioreagent and/or improve the medium'"'"'s capability of drying to a substantially water resistant or insoluble layer. The immobilization medium provided by the instant invention is in the form of a homogeneous liquid suspension.

Claims

1. An immobilization medium comprising:

a. a bioreagent immobilized to a solid phase, wherein said solid phase is comprised of particles with a polymer absorbed thereon, wherein said adsorbed polymer is selected from the group consisting of quaternary ammonium polymers, tertiary amino polymers, secondary amino polymers, and primary amino polymers; and

b. a latex resin, wherein said immobilized bioreagent is evenly dispersed in said latex resin;

wherein said immobilization medium is a substantially homogeneous dispensable mixture.

2. The medium of claim 1 wherein said bioreagent is selected from the group consisting of analytes and analyte analogs.

3. The medium of claim 1 wherein said bioreagent is an enzyme.

4. The medium of claim 1 wherein said bioreagent is an antibody or an antibody fragment.

5. The medium of claim 1 wherein said bioreagent is a nucleic acid.

6. The medium of claim 1, wherein said particles comprise a material selected from the group consisting of inorganics, organics, carbohydrates and combination of two or more inorganics, organics, and carbohydrates.

7. The medium of claim 6 wherein said particle are selected from the group consisting of silica particles, agarose particles, cellulose particles, derivatized cellulose particles, carbon particles, platinized carbon particles, graphite particles, platinized graphite particles, aluminum oxide particles, aluminosilicate particles, titanium dioxide particles, platinum group metal oxide particles, metalloporphyrin carbon particles, metalloporphyrin graphite particles, and combinations of two or more silica particles, agarose particles, cellulose particles, derivatized cellulose particles, carbon particles, platinized carbon particles, graphite particles, platinized graphite particles, aluminum oxide particles, aluminosilicate particles, titanium dioxide particles, platinum group metal oxide particles metalloporphyrin carbon particles, and metalloporphyrin graphite particles.

8. The medium of claim 7 wherein said particles are finely divided.

9. The medium of claim 6, wherein said organics further comprise synthetic organics.

10. The medium of claim 1 wherein said latex resin is selected from the group consisting of an acrylic latex, a styrene acyrlic latex, a vinyl acetate latex, a polyurethane latex and combinations of two or more of an acrylic latex, a styrene acyrlic latex, a vinyl acetate latex and a polyurethane latex.

11. The medium of claim 1 further comprising means for modifying the chemical and physical properties of the immobilization medium.

12. The medium of claim 11 wherein said modifying means are selected from the group consisting of plasticizers, thickeners, film forming agents, stabilizers, dispersing agents, defoaming agents, electrochemically active agents and combinations of two or more plasticizers, thickeners, film forming agents, stabilizers, dispersing agents, defoaming agents, and electrochemically active agents.

13. The medium of claim 1, wherein said bioreagent is selected from the group consisting of detectable moieties and specific binding members.

14. The medium of claim 1, wherein said bioreagent is covalently immobilized to said solid phase.

15. The medium of claim 1, wherein said bioreagent is ionically immobilized to said solid phase.