Method of synthesis of plurality of compounds in parallel using a partitioned solid support
First Claim
1. A method of synthesizing a plurality of proteins or oligonucleotides in parallel which comprises,providing a substrate having a plurality of wells, each well connected to a plurality of reservoirs for reagents or solvents, which reservoirs are formed in the substrate, the reservoirs connected to said wells by one or more channels, wherein the channel(s) connected to a particular reservoir do not intersect with channel(s) connected to other reservoirs so that reagents and solvents are controllably mixed in the wells;
- moving reagents or solvents in sequence from said reservoirs along said channels and into and out of said wells by means of electrokinetic pumps each connected to a driver means; and
reacting said reagents to form a plurality of proteins or oligonucleotides.
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Accused Products
Abstract
Methods are provided for synthesizing a plurality of compounds in parallel. The methods use cross-over channels for moving samples from a reservoir of starting materials into a plurality of wells. The movement is achieved, for example, using an electrokinetic pump.
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Citations
24 Claims
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1. A method of synthesizing a plurality of proteins or oligonucleotides in parallel which comprises,
providing a substrate having a plurality of wells, each well connected to a plurality of reservoirs for reagents or solvents, which reservoirs are formed in the substrate, the reservoirs connected to said wells by one or more channels, wherein the channel(s) connected to a particular reservoir do not intersect with channel(s) connected to other reservoirs so that reagents and solvents are controllably mixed in the wells; -
moving reagents or solvents in sequence from said reservoirs along said channels and into and out of said wells by means of electrokinetic pumps each connected to a driver means; and reacting said reagents to form a plurality of proteins or oligonucleotides. - View Dependent Claims (2, 3, 7, 8, 9, 10)
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4. A method of synthesizing a plurality of different compounds in parallel which comprises,
providing a substrate having a plurality of wells, each well connected to a plurality of reservoirs for reagents or solvents, which reservoirs are formed in the substrate, the reservoirs connected to said wells by one or more channels, wherein the channel(s) connected to a particular reservoir do not intersect with channel(s) connected to other reservoirs so that reagents and solvents are controllably mixed in the wells; -
adding a starting material having a protecting group to plurality of the wells; removing the protecting group from the starting material; and adding a coupling agent to each well from a reservoir by means of a channel connected to said reservoir and to said well;
thereby producing a plurality of different compounds. - View Dependent Claims (5, 6, 11, 12, 13, 14, 15, 16)
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17. A method of synthesizing a plurality of different compounds comprising:
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a) providing substrate having an array of at least one hundred wells that each have a connection to two or more reservoirs, which reservoirs are formed in the substrate, the connections being provided by channels, which channels are formed in the substrate, wherein the channels connecting the wells to reservoirs would intersect but for crossovers formed by a plurality of channels, whereby a first portion of each such a channel is situated horizontally at a first vertical level within the substrate, at an endpoint of the first portion a second portion of the channel extends vertically to a second vertical level within the substrate and connects to a third portion of the channel that extends the channel in a horizontal dimension, and whereby the change in the vertical level of the channel allows the channel to avoid intersecting one or more other channels within the substrate; b) adding a starting material having a protecting group to the wells; c) removing the protecting group from the starting material; and d) adding a coupling agent to each well from a reservoir by means of a channel connected to the reservoir and the well, thereby producing a plurality of different compounds. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24)
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Specification