Methods and apparatus for electrochemical measurements
First Claim
1. A non-enzymic method of selectively determining the content of reducing sugars only in an aqueous solution, comprising forming a thin layer having a thickness of less than about 1 mm of a solution to be tested in an alkaline environment containing a soluble redox mediator and in the absence of enzymes catalyzing reactions of reducing sugars;
- determining electrochemically the amount of reduction product of the redox mediator; and
determining from the amount of reduction product the content of reducing sugars in the aqueous solution.
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Abstract
A method for the electrochemical determination of the reducing sugars content of an aqueous solution in a thin layer electrochemical measuring device, preferably a capillary fill device, wherein an inner surface of the device is coated with a soluble redox mediator, the device including means for producing an alkaline environment within the device. In a preferred method the redox mediator comprises potassium ferricyanide providing ferricyanide ions in saturated solution and lithium hydroxide providing hydroxide ions in an excess amount of from 1 to 3 molar. The invention also provides a capillary fill device having a guard electrode between the reference and working electrodes.
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Citations
17 Claims
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1. A non-enzymic method of selectively determining the content of reducing sugars only in an aqueous solution, comprising forming a thin layer having a thickness of less than about 1 mm of a solution to be tested in an alkaline environment containing a soluble redox mediator and in the absence of enzymes catalyzing reactions of reducing sugars;
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determining electrochemically the amount of reduction product of the redox mediator; and determining from the amount of reduction product the content of reducing sugars in the aqueous solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification