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Method and compositions for identification of species in a sample using type II topoisomerase sequences

  • US 5,645,994 A
  • Filed: 06/06/1995
  • Issued: 07/08/1997
  • Est. Priority Date: 07/05/1990
  • Status: Expired due to Term
First Claim
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1. A method of selectively amplifying DNA segments of one or more species of organisms in a sample, comprising the steps of:

  • providing a database containing a plurality of reference sequences each comprising a subunit sequence of a selected signature region of a macromolecule selected from the group consisting of;

    type II topoisomerase enzymes and homologues of type II topoisomerase enzymes;

    each of the reference sequences being specific to a different individual species of a chosen group, and the selected macromolecule further having first and second conserved regions adjacently flanking the selected signature region;

    providing a universal primer composition comprising a primer pair constructed to bind to a DNA encoding the macromolecule;

    making an extract of DNA molecules from a sample; and

    using the primer composition to selectively amplify DNA segments of the signature region in the extract to produce amplified DNA segments.

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