High-affinity oligonucleotide ligands to the tachykinin substance P
First Claim
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1. A method of identifying nucleic acid ligands to Substance P (SP), comprising:
- a) preparing a candidate mixture of nucleic acids;
b) contacting said candidate mixture of nucleic acids with SP, wherein nucleic acids having an increased affinity to SP relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and
d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with said increased affinity to SP, whereby nucleic acid ligands of SP may be identified.
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Abstract
Methods are described for the identification and preparation of nucleic acid ligands to Substance P (SP). Included in the invention are specific RNA ligands to SP identified by the SELEX method.
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Citations
9 Claims
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1. A method of identifying nucleic acid ligands to Substance P (SP), comprising:
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a) preparing a candidate mixture of nucleic acids; b) contacting said candidate mixture of nucleic acids with SP, wherein nucleic acids having an increased affinity to SP relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with said increased affinity to SP, whereby nucleic acid ligands of SP may be identified. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for identifying nucleic acid ligands from a candidate mixture of nucleic acids, said nucleic acid ligands being a ligand of a given target molecule comprising:
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a) contacting said candidate mixture with the target molecule, wherein nucleic acids having increased affinity to the target molecule may be partitioned from the remainder of the candidate mixture; b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; c) amplifying the increased affinity nucleic acids to yield a ligand-enriched mixture of nucleic acids; d) preparing a second candidate mixture of nucleic acids by mutagenizing the ligand-enriched mixture of nucleic acids, whereby nucleic acid ligands of the given target molecule may be identified. - View Dependent Claims (9)
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Specification