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High-affinity oligonucleotide ligands to the tachykinin substance P

  • US 5,648,214 A
  • Filed: 09/09/1994
  • Issued: 07/15/1997
  • Est. Priority Date: 06/11/1990
  • Status: Expired due to Term
First Claim
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1. A method of identifying nucleic acid ligands to Substance P (SP), comprising:

  • a) preparing a candidate mixture of nucleic acids;

    b) contacting said candidate mixture of nucleic acids with SP, wherein nucleic acids having an increased affinity to SP relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;

    c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and

    d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with said increased affinity to SP, whereby nucleic acid ligands of SP may be identified.

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