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Expression of functional antibody fragments

  • US 5,648,237 A
  • Filed: 05/03/1995
  • Issued: 07/15/1997
  • Est. Priority Date: 09/19/1991
  • Status: Expired due to Term
First Claim
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1. A method for the production and purification of a Fab'"'"' having a hinge region comprising the steps of:

  • (a) expressing nucleic acid encoding the Fab'"'"' in a microbial host cell comprising said nucleic acid such that the Fab'"'"' is secreted to the periplasmic space of the host cell; and

    (b) recovering the Fab'"'"' from said host cell, wherein the amount of Fab'"'"' recovered from the host cell is higher than the amount of F(ab'"'"')2 recovered from the host cell and the recovered Fab'"'"' is able to be covalently bonded to a second Fab'"'"' to form a functional F(ab'"'"')2.

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