Method and apparatus for conducting tests

• US 5,650,125 A
• Filed: 04/12/1995
• Issued: 07/22/1997
• Est. Priority Date: 10/14/1992
• Status: Expired due to Term

• Current Assignee: Andrew George Bosanquet
• Original Assignee: Andrew George Bosanquet
• Inventors: Bosanquet, Andrew George
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US08/411,827
• Time in Patent Office: 832 Days
• Field of Search: 422/72, 422/82.05, 422/82.09, 422/99, 422/102, 422/104, 436/63, 436/165, 436/174, 436/177, 436/178, 435/287.1, 435/287.7, 435/288.4, 435/288.5, 435/288.7, 435/307.1, 435/809
• US Class Current: 422/548
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 3/50855 using modular assemblies of strips or of individual wells
• CPC Class Codes: B01L 9/06 Test-tube stands; Test-tube holders
• CPC Class Codes: G01N 1/2813 Producing thin layers of samples on a substrate, e.g. smearing, spinning-on G01N1/30 takes precedence
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The apparatus includes a receptacle member in the form of at least one row of receptacles for receiving cell samples, each having a closed end and an open end, and a holder adapted to receive the receptacle member in a predetermined orientation and having an abutment for determining the position of a microscope slide relative to the receptacle to allow use of the receptacle member in a centrifuge for sedimentation of the samples through the open ends of the receptacles onto the microscope slide at predetermined positions. The preferred material for the receptacle member is one having a high degree of optical transparency whilst being substantially non-adherent to cells especially tumour cells and several suitable materials are described.

Claims

1. In an apparatus for use in forming a plurality of cell samples on a receiving member for microscopic examination, comprising a plurality of receptacles for samples containing cells to be investigated, each receptacle having an open end and a closed end, a receiving member for said receptacles, an absorbent member defining a plurality of opening arranged for alignment with said open ends of said receptacles for interposition between said open ends of said receptacles and said receiving member, a holder for said receptacles allowing assembly to said receiving member with the interposition of said absorbent member, and securing means for holding said receiving member, said absorbent member, said holder and said receptacles together as an assembly with said open ends of said receptacles in contact with said absorbent member and each open end in register with a respective one of said openings therein whereby, upon centrifugation, supernatant liquid is absorbed by said absorbent member through edges defining said openings therein without leakage around said open end of each receptacle and the respective opening in the absorbent member, wherein the improvement comprises:

said receptacles are joined together as a unitary receptacle member comprising at least one row of said receptacles, the internal surface of each said receptacle is at least substantially non-adherent to cell material whereby to allow at least said cells in a said sample to be transferred by centrifugation from each said receptacle through said open end thereof onto said receiving member, said holder receives said unitary receptacle member solely in a single predetermined orientation and has abutment means for abutment by said receiving member whereby to determine the relative position of said unitary receptacle member and said receiving member in an assembly constituted by said holder, said unitary receptacle member received therein, said absorbent member located with said openings in register with said open ends of said receptacles and said receiving member held together by said securing means, and wherein the closed ends of said receptacles are defined by bottom walls lying in a common plane and at least said bottom walls of each receptacle being composed of a material substantially transparent to light to allow the sample in the receptacle to be assayed by an optical technique involving the transmission of light through the sample and the bottom wall.

2. The apparatus of claim 1 wherein said receptacles of said unitary receptacle member are spaced at a substantially constant pitch from each other in said at least one row, and the receptacle at a first end of the at least one row is closer to an adjacent end of said receptacle member than the receptacle at the other end of the at least one row is to an adjacent end of said receptacle member.

3. The apparatus of claim 1, wherein adjacent receptacles in said unitary receptacle member are joined together by a web holding them in a predetermined spaced relationship.

4. The apparatus of claim 1, wherein the center-to-center spacing of adjacent receptacles in said unitary receptacle member is one of substantially 4.5 mm or 9 mm.

5. The apparatus of claim 1, wherein the unitary receptacle member is formed with a lower transverse flange acting as a base to hold the member in an upright orientation with said open ends of the receptacles uppermost.

6. The apparatus of claim 1, wherein the unitary receptacle member comprises a plurality of rows and columns in an array, and at least one row or column is separable from said array as a single unit.

7. The apparatus of claim 1, wherein said holder has a support face, said receptacles have radially projecting flanges at said open ends, said flanges each having an annular end face defining the opening of said open end for contact with said absorbent member in said assembly and a shoulder for abutment with said support face to space each said end face from said support face, and wherein said receptacle member, said holder, said absorbent member and said securing means being so dimensioned that said end faces of said receptacles contact said absorbent member in said assembly without so compressing said absorbent member as to form a seal around said open cads of said receptacles.

8. The apparatus of claim 7 wherein the flanges on said receptacles are formed of or coated with an opaque material.

9. The apparatus of claim 7, wherein said abutment means on said holder comprises a shoulder on said support face against which said receiving member is engageable.

10. The apparatus of claim 7, wherein said absorbent member defines a further opening in addition to and offset from said plurality of openings and said support face on said holder has a locating projection upstanding therefrom for engagement with said further opening to locate said absorbent member in a predetermined position relative to said holder.

11. A kit for use in forming a plurality of cell samples on a receiving member for microscopic examination, comprising:

a plurality of receptacles for receiving media samples containing cell samples to be investigated, each receptacle having an open end and a closed end;

a holder for said plurality of receptacles;

a receiving member for receiving said cell samples from said receptacles;

an absorbent member having edges defining a plurality of openings therein arranged for alignment with said open ends of said receptacles for interposition between said open ends of said receptacles and said receiving member; and

securing means for securing said holder with said receptacles therein, said absorbent member and said receiving member together as an assembly with said open ends of said receptacles in contact with said absorbent member and each open end in register with a respective one of said openings therein whereby, upon centrifugation, supernatant liquid is absorbed by said absorbent member through edges defining said openings therein without leakage, around a junction between said open end of each receptacle and the respective opening in said absorbent member, wherein said receptacles are joined together as a unitary receptacle member comprising at least one row of said receptacles, wherein each said receptacle has an internal surface which is at least substantially non-adherent to cell material whereby to allow at least said cell sample to be transferred by centrifugation from said receptacle through said open end thereof onto said receiving member, wherein said holder receives said unitary receptacle member solely in a single predetermined orientation and has abutment means for abutment by said receiving member whereby to determine the relative position of said unitary receptacle member and said receiving member in said assembly and thereby determine the positions at which said cell samples are formed on said receiving member in use and wherein the closed ends of said receptacles are defined by bottom walls lying in a common plane and at least said bottom walls of each receptacle being composed of a material substantially transparent to light to allow the sample in the receptacle to be assayed by an optical technique involving the transmission of light through the sample and the bottom wall.

12. A kit as claimed in claim 11, wherein said holder has a support face, said receptacle member is received in said holder with said open ends of said receptacles adjacent said support face, said support face has at least one locating projection upstanding therefrom and said absorbent member defines a cooperating aperture offset from said plurality of openings therein for engagement by said at least one locating projection, said at least one locating projection and said cooperating aperture being so positioned as to locate said absorbent member with said plurality of openings in register with said open ends of the receptacles in said assembly.

13. A kit as claimed in claim 12, wherein said receiving member comprises a microscopic slide and said holder support face has an upstanding shoulder for abutment by said microscopic slide to locate said microscopic slide in a predetermined position for receiving said cell samples.

14. A kit as claimed in claim 11, wherein some of said receptacles contain a drug for use in testing said cell samples.

15. A kit as claimed in claim 14, wherein some of said receptacles contain a drug for use in testing said cell samples and others of said receptacles contain a toxic substance to serve as positive controls for said testing.

16. A kit as claimed in claim 14, wherein some of said receptacles contain no added substance to serve as negative controls for said testing.