Process for preparing artificial skin with biocompatible perforated membranes

• US 5,650,164 A
• Filed: 03/14/1994
• Issued: 07/22/1997
• Est. Priority Date: 06/01/1990
• Status: Expired due to Term

• Current Assignee: Fidia S.P.A.
• Original Assignee: Fidia S.P.A.
• Inventors: Della Valle, Francesco, Romeo, Aurelio, Calderini, Gabriella, Rastrelli, Alessandro
• Primary Examiner(s): Phelan, D. Gabrielle
• Application Number:
  US08/209,626
• Time in Patent Office: 1,226 Days
• Field of Search: 424/448, 424/449, 424/422, 424/425, 523/113, 623/15
• US Class Current: 424/422
• CPC Class Codes: A61F 2/105 Skin implants, e.g. artificial skin
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61L 27/60 Materials for use in artificial skin
• CPC Class Codes: C12N 2533/70 Polysaccharides
• CPC Class Codes: C12N 2533/80 Hyaluronan
• CPC Class Codes: C12N 2539/10 Coating allowing for selective detachment of cells, e.g. thermoreactive coating
• CPC Class Codes: C12N 5/0062 General methods for three-dimensional culture
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0629 Keratinocytes; Whole skin
• CPC Class Codes: Y10T 428/24273 including aperture

Abstract

The invention relates to biocompatible membranes constructed of materials of natural, synthetic or semisynthetic origin, and having a thickness of between 10 and 500 μ, characterised by containing an ordered series of holes of a constant size between 10 and 1000 μ, separated from each other by a constant distance of between 50 and 1000 μ, and obtained by perforation by mechanical, thermal laser or ultraviolet radiation means, they being suitable for use as a support for the in vitro growth of epithelial cells, the invention also relating to the artificial skin obtained thereby and its use in grafts.

Claims

1. A process for preparing artificial skin, comprising:

a) perforating, via a screening system using a mechanical or laser perforation device, a continuous biocompatible membrane that can support the in vitro cultivation of epithelial cells comprising a natural, synthetic, or semisynthetic material having a thickness of between 10 and 500 μ

m thereby obtains an ordered series of holes having a diameter between 10 and 1000 μ

m, separated from each other by a constant distance of between 50 and 1000 μ

m, wherein epithelial cells can be cultured in vitro within said holes and on both sides of said membrane, and

b) seeding said perforated biocompatible membrane of step a) with autologous or heterologous epithelial cells in the active proliferation stage and cultivating said epithelial cells thereon in vitro.

2. The process of claim 1, wherein said mechanical perforation device is a punch.

3. The process of claim 1, wherein said laser perforation device is a UV radiation laser.

4. The process of claim 1 wherein, wherein said epithelial cells are keratinocytes.

5. The process of claim 1, wherein the diameter of said holes is between 40 and 70 μ

m.

6. The process of claim 1, wherein the distance between said holes is 80 μ

m.

7. The process of claim 1, wherein said membrane has a thickness between 20 and 40 μ

m.

8. The process of claim 1, wherein said material of natural origin is selected from the group consisting of collagen, a coprecipitate of collagen and a glycosaminoglycan, cellulose, a gelled polysaccharide, a natural rubber, a mixture thereof, and a mixture thereof with a synthetic or semisynthetic polymer, in the presence of a precipitating or gelling agent.

9. The process of claim 8, wherein said gelled polysaccharide is selected from the group consisting of chitin, chitosan, pectin, pectic acid, agar, agarose, xanthan gum, gellano, alginic acid, an alginate, a polymannan, a polyglucan, and a starch.

10. The process of claim 1, wherein said material of synthetic origin is selected from the group consisting of polylactic acid, polyglycolic acid, a copolymer thereof, a polydioxanone, a polyphosphazene, a polysulphone, and a polyurethane.

11. The process of claim 1, wherein said material of semisynthetic origin is selected from the group consisting of collagen crosslinked with a crosslinking agent and alginic acid, and a hyaluronic acid ester.

12. The process of claim 11, wherein said biocompatible membrane consists of the 100% benzyl ester of hyaluronic acid.

13. The process of claim 11, wherein said crosslinking agent is selected from the group consisting of a dialdehyde, a dialdehyde precursor, a bicarboxylic acid, a halide of a bicarboxylic acid, and a diamine.

14. The process of claim 1, wherein said material of synthetic origin is selected from the group consisting of silicone, a silane rubber, a siloxane rubber, a fluoropolymer, a polystyrene, a vinyl polychloride, a polyacrylate, a polyhydroxyacrylate, a polyhydroxymethacrylate, a carboxyvinyl polymer, a maleic anhydride polymer, a polyvinylchloride, a polyvinylalcohol, polyethylene, and polypropylene.

15. The process of claim 14, wherein said fluoropolymer is selected from the group consisting of polyfuoroethylene, polyfluoropropylene, and a polyfluroether.

16. The process according to claim 1 further comprising cryopreserving said artificial skin.