System for growing and manipulating tissue cultures using 96-well format equipment

• US 5,650,323 A
• Filed: 06/26/1991
• Issued: 07/22/1997
• Est. Priority Date: 06/26/1991
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: CoStar Group Inc.
• Inventors: Root, David
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Thornton, Krisanne M.
• Application Number:
  US07/721,250
• Time in Patent Office: 2,218 Days
• Field of Search: 435/284, 435/285, 435/300, 435/301, 435/311, 435/297, 435/298, 422/101, 422/102, 210/238, 210/406, 210/450, 210/482, 220/800, 220/803, 220/806, 220/807
• US Class Current: 435/284.1
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence

Abstract

A laboratory research tool for conducting a variety of procedures including cell and tissue culture techniques includes a multi-well cluster plate, a filter plate that has one or more filter wells that extend into the wells of the cluster plate, a cover and a reservoir that can be used with auxiliary equipment designed for use with standard 96-well format cluster plates. The system allows multiple tissue samples to be grown and manipulated simultaneously.

Claims

1. A multi-well cluster system designed for use with auxiliary equipment that loads and unloads precise volumes of liquid and/or reads the light transmitted through or emanating from each of the wells in a standard format 96-well plates comprising:

a cluster plate having a matrix of wells, fewer in number than 96, forming at least one row of wells wherein the wells are spaced apart, center to center, a distance which is a whole number multiple of the distance between adjacent wells in the 96 well standard format and wherein the cluster plate wells are each aligned with a different wells in the standard format enabling the cluster plate to be used with said auxiliary equipment,

the diameters of each of the wells in the cluster plate being greater than the center to center distance of adjacent wells in the standard 96-well.

2. A multi-well cluster system as described in claim 1 wherein the matrix of wells compriseswells with their centers arranged in 12 parallel rows of 4 wells each and with the centers of the wells in each row being staggered with respect to the centers of the wells in the adjacent rows.

3. A multi-well system as defined in claim 2 wherein

the cluster plate has overall length and width dimensions equal to the corresponding dimensions of a 96-well standard format plate.

4. A multi-well system as defined in claim 1 wherein

the cluster plate has overall length and width dimensions equal to the corresponding dimensions of a 96-well standard format plate.

5. A multi-well cluster system as described in claim 3 wherein the system includes a second plate having a plurality of wells each having a solid side wall and a bottom wall made of porous material, said wells in the second plate being spaced apart and sized to fit into separate wells in the cluster plate.

6. A multi-well cluster system

as described in claim 1 wherein the system includes a second plate having a plurality of wells each having a solid side wall and a bottom wall made of porous material, said wells in the second plate being spaced apart and sized to fit into separate wells in the cluster plate.

7. A multi-well cluster system as defined in claim 6 wherein the wells in the porous plate are equal in number to and are in the same format as the wells in the cluster plate.

8. A multi-well cluster system as defined in claim 6 where in the cluster plate and porous plate each have 48 wells.

9. A multi-well cluster system as defined in claim 6 wherein the porous plate has half the number of wells as are in the cluster plate.

10. A multi-well cluster system as defined in claim 6 wherein the cluster plate has 48 wells.

11. A multi-well cluster system as defined in claim 10 wherein the porous plate has 24 wells.

12. A multi-well cluster system as defined in claim 10 wherein the porous plate has 12 wells.

13. A multi-well cluster system as defined in claim 10 wherein the porous plate has 8 wells.

14. A multi-well cluster system as defined in claim 10 wherein the porous plate has 4 wells.

15. A multi-well cluster system as defined in claim 10 wherein the porous plate has one well.

16. In a system as defined in claim 6, the diameter of each of said wells in the second plate being equal to the diameter of the wells in a standard 96-well cluster plate.

17. A system as defined in claim 6 wherein, a reservoir is provided for containing media wherein said reservoir is constructed to receive simultaneously all of the wells of the second plate.

18. In a system as defined in claim 6, said wells of the second plate being connected together by frangible connecting means so that they may be selectively broken away from other wells to which they are connected.

19. A system as defined in claim 6, wherein means are operatively connected to the cluster plate and the second plate for positioning the two together so that the wells of the second plate are aligned with the wells in the cluster plate.

20. A system as defined in claim 19, wherein said means operatively connected to the cluster plate and second plate establish a selected depth the wells of the second plate may extend into the cluster plate wells.

21. A multi-well cluster system as defined in claim 19 wherein the wells in the second plate are equal in number to and are in the same format as the wells in the cluster plate.

22. A multi-well cluster system as defined in claim 19 wherein the cluster plate has 48 wells.

23. A system as defined in claim 19, wherein means are provided in the second plate for providing access to the wells in the cluster plate and second plate while the second plate is positioned with its wells extending into the cluster plate wells.

24. A multi-well system as defined in claim 1 wherein the cluster plate has 48 wells.

25. A multi-well system as defined in claim 4 wherein

the center to center distance between adjacent wells in the same row is twice the center to center distance between adjacent wells in the 96-well format.

26. A multi-well cluster system as defined in claim 5 wherein the second plate has half the number of wells as are in the cluster plate.