Method and device for determination of parameters of individual microparticles

• US 5,650,847 A
• Filed: 06/14/1995
• Issued: 07/22/1997
• Est. Priority Date: 06/14/1995
• Status: Expired due to Term

• Current Assignee: Erkki Soini
• Original Assignee: Erkki Soini
• Inventors: Chernyshev, Andrei Vitalievich, Maltsev, Valeri Pavlovich
• Primary Examiner(s): Font, Frank G.
• Assistant Examiner(s): MERLINO, AMANDA H
• Application Number:
  US08/490,454
• Time in Patent Office: 769 Days
• Field of Search: 356/336, 356/128, 356/73, 356/337, 356/343
• US Class Current: 356/336
• CPC Class Codes: G01N 15/0205 by optical means
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry

Abstract

A method and device for determination of absolute values of the size and the refractive index of individual microparticles. An optical scanning device for measurement of angular scattering pattern from individual microparticles is described. The light scatter is measured in different angles from moving particles inside the scanning optical system and the values and the angular locations of minima and maxima of the scattering pattern are registered. The size and the refractive index of individual microparticles are calculated from the registered minima and maxima using an empirical equation. An empirical equation has been derived from statistical analysis of angular scattering patterns. These patterns have been calculated on the basis of Mie scattering theory.

Claims

1. A device for determining the size and refractive index of individual microparticles contained within a liquid sample, comprising in operational relationship:

a laser capable of emitting a laser beam along an axis,

a capillary flow channel placed in coaxial relationship with said laser in said axis,

a spherical reflector having an optical axis in coaxial relationship with said capillary flow channel,

a lens,

a pinhole, and

a photodetector,

arranged such that in operation laser light impinges upon individual microparticles in the capillary flow channel and is scattered onto said spherical reflector, which reflects the laser light to said lens, which focusses the reflected laser light through the pinhole onto said photodetector.

2. The device of claim 1, further comprising a plane reflector such that in operation laser light impinges upon individual microparticles in the capillary flow channel and is scattered onto said spherical reflector, which reflects the laser light to said plane reflector and lens, which focusses the reflected laser light through the pinhole onto said photodetector.

3. A method for determining the size and refractive index of individual microparticles contained within a liquid sample, comprising:

providing a laser capable of emitting a laser beam along an axis, a capillary flow channel in coaxial relationship with said laser in said axis, a spherical reflector having an optical axis in coaxial relationship with said capillary flow channel, a lens, a pinhole, and a photodetector,

placing a liquid sample containing microparticles in said capillary flow channel;

illuminating said liquid sample with light from said laser;

focussing the light scattered by said microparticles onto said photodetector by means of said spherical reflector, said lens and said pinhole,

measuring the scattered light intensity at different angles at different times such that minima and maxima of a scattering pattern of the microparticles are recorded.

4. The method of claim 3, further providing a plane reflector such that light reflected by said spherical reflector is reflected onto said plane reflector and reflected therefrom on said lens.

5. The method according to claim 3 wherein the absolute values of diameter and refractive index of individual microparticles are calculated from their light scattering patterns using an empirical equation derived from statistical analysis of angular scattering patterns or from direct comparison of the theoretical and the experimental light scattering patterns using dot product, simple correlation, Pearson'"'"'s correlation or neural network methods.