Methods of use of uncoated gel particles
First Claim
1. A method of implanting a living donor cell into a host animal, wherein said animal is a mammal larger than a rat or mouse, with minimal inflammatory response or rejection of the donor cell by the host animal, said method comprising the steps ofobtaining an uncoated particle consisting essentially of a biocompatible, temperature-independent gel that encapsulates the living donor cell, wherein said uncoated particle provides a molecular weight cutoff that prevents host animal immune cells from entering the particle, and does not prevent entry of host animal IgG and complement into said particle, wherein said particle has a diameter of 700-6,000 microns, andimplanting the uncoated particle into the host animal.
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Abstract
The invention covers a method of implanting a living donor cell into a host animal without inflammatory response or rejection of the donor cell by the host animal, by obtaining an uncoated particle of a biocompatible, temperature-independent gel that encapsulates the living donor cell, wherein the uncoated particle provides a molecular weight cutoff that prevents host animal immune cells from entering the particle, yet does not have to prevent entry of host animal IgG and complement into the particle, and implanting the uncoated particle into the host animal.
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Citations
64 Claims
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1. A method of implanting a living donor cell into a host animal, wherein said animal is a mammal larger than a rat or mouse, with minimal inflammatory response or rejection of the donor cell by the host animal, said method comprising the steps of
obtaining an uncoated particle consisting essentially of a biocompatible, temperature-independent gel that encapsulates the living donor cell, wherein said uncoated particle provides a molecular weight cutoff that prevents host animal immune cells from entering the particle, and does not prevent entry of host animal IgG and complement into said particle, wherein said particle has a diameter of 700-6,000 microns, and implanting the uncoated particle into the host animal.
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25. A method of implanting a living donor cell into a host animal, wherein said animal is a mammal larger than a rat or mouse, with inflammatory response or rejection of the donor cell by the host animal, said method comprising the steps of
suspending the living donor cell in a liquid medium, said medium consisting essentially of water and a biocompatible, temperature-independent liquid gel, forming a droplet of said liquid medium that contains at least one living cell, solidifying said droplet to form a gel particle that encapsulates the living cell, whereby no outer coating is formed on said particle, and wherein said uncoated particle provides a molecular weight cutoff that prevents host animal immune cells from entering the particle, and does not prevent entry of host animal IgG and complement into said particle, wherein said particle has a diameter of 700-6,000 microns, and implanting the uncoated particle into the host.
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29. A method of treating a disease in a patient, wherein said patient is a mammal larger than a rat or mouse, caused by a deficient production of a substance in the patient, said method comprising the steps of
obtaining an uncoated particle consisting essentially of a biocompatible, temperature- independent gel that encapsulates a living donor cell that secretes said substance, wherein said uncoated particle provides a molecular weight cutoff that prevents patient immune cells from entering the particle, and does not prevent entry of patient IgG and complement into said particle, wherein said particle has a diameter of 700-6,000 microns, and implanting the uncoated particle into the patient in a location and in a manner that allows the living cell to remain physiologically active and secrete said substance into the patient to treat said disease.
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42. An in vivo method of culturing a living cell, said method comprising the steps of
encapsulating the living cell in an uncoated particle consisting essentially of a biocompatible, temperature-independent gel, inserting said uncoated particle into an animal, and allowing said animal to thrive, thereby culturing the cell wherein said animal is a mammal larger than a rat or mouse, wherein said particle has a diameter of 700-6,000 microns and wherein said uncoated particle provides a molecular weight cutoff that prevents host animal immune cells from entering the particle, and does not prevent entry of host animal IgG and complement into said particle.
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43. An in vitro method of culturing a living cell, said method comprising the steps of
encapsulating the living cell in an uncoated particle consisting essentially of a biocompatible, temperature-independent gel, wherein said particle has a diameter of 700-6,000 microns and wherein said uncoated particle provides a molecular weight cutoff that prevents host animal immune cells from entering the particle, and does not prevent entry of host animal IgG and complement into said particle placing said uncoated particle into a medium including nutrients and oxygen, and maintaining a sufficient amount of nutrients and oxygen in said medium to allow the cell to thrive, thereby culturing the cell.
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44. A method of manufacturing uncoated, temperature-independent gel particles containing living cells consisting of the steps of
suspending the living cells in a liquid medium, said medium consisting essentially of water and a biocompatible, temperature-independent, liquid gel, forming a droplet of said liquid medium, solidifying said droplet to form a gel particle that encapsulates the living cells, whereby no outer coating is formed on said particle, wherein said particle has a diameter of 700-6,000 μ - m and wherein said uncoated particle provides a molecular weight cutoff that prevents host animal immune cells from entering the particle, and does not prevent entry of host animal IgG and complement into said particle, and
storing said gelled uncoated particles in a nutrient medium to maintain the viability of the living cells.
- m and wherein said uncoated particle provides a molecular weight cutoff that prevents host animal immune cells from entering the particle, and does not prevent entry of host animal IgG and complement into said particle, and
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46. A method of implanting a living donor cell into a host animal, wherein said animal is a mammal larger than a rat or mouse, with minimal inflammatory response or rejection of the donor cell by the host animal, said method comprising the steps of
obtaining an uncoated particle consisting essentially of a biocompatible, temperature- independent gel that encapsulates the living donor cell, wherein said uncoated particle provides a molecular weight cutoff that prevents host animal immune cells from entering the particle, and does not prevent entry of host animal IgG and complement into said particle, implanting the uncoated particle into the host animal, and administering a drug to the host animal at a dosage effective to inhibit fibrosis and inflammation of the uncoated particle, but at a dosage lower than that required to achieve immunosuppression when the donor cell is implanted into the host animal without encapsulation.
Specification