Probes comprising fluorescent nucleosides and uses thereof
First Claim
1. A fluorescent nucleoside, or structural analog thereof, having either of the following structures:
- ##STR2## wherein X1, X2, X3, X4, X5, and X6 =N, O, C, S, or Si, wherein at least one of X1, X2, X3, X4, X5, or X6 =N;
R4 is a reactive group derivatizable with a detectable label wherein said reactive group is selected from the group consisting of NH2, SH, ═
O, and optionally, a linking moiety selected from the group consisting of an amide, a thioether, a disulfide, a combination of an amide a thioether or a disulfide, R1 --(CH2)x --R2 and R1 -R2 --(CH2)x --R3 wherein x is an integer from 1 to 25 inclusive, and R1, R2, and R3 are H, OH, alkyl, acyl, amide, thioether, or disulfide, and wherein said detectable label is selected from the group consisting of radioisotopes, fluorescent or chemiluminescent reporter molecules, antibodies, haptens, biotin, photobiotin, digoxigenin, fluorescent aliphatic amino groups, avidin, enzymes, and acridinium;
R5 is H or part of an etheno linkage with R4 ;
R6 is H, NH2, SH, or ═
O;
R8 and R9 are hydrogen, methyl, bromine, fluorine, or iodine;
alkyl or aromatic substituents, or an optional linking moiety selected from the group consisting of an amide, a thioether, a disulfide linkage, and a combination thereof;
R10 is hydrogen, an acid-sensitive/base-stable blocking group, or a phosphorous derivative;
R11 =R13 =H;
R12 is hydrogen, OH, 3'"'"' amino, 3'"'"'-azido, 3'"'"'-thiol, 3'"'"'-unsaturated or a 3'"'"'-phosphorous derivative; and
R14 is H, OH, or OR3 where R3 is a reactive group, protecting group, or additional fluorophore;
provided that excluded from such compound is any purine-like compound in which;
(i) X1=X4=C;
X2=X3=N;
R4=NH2;
R5=R8 which is absent;
R6=H;
R9 is H or is absent;
R10=H; and
R12=R14=OH;
or(ii) X1=C;
X2=X3=X4=N;
R4=NH2 or H;
R5=R8 which is absent;
R6=NH2;
R9 is H or is absent;
R10=H; and
R12=R14=OH;
or(iii) R4 and R5 in combination form an etheno linkage;
R6=R8=H;
R9 is absent;
X1=X3=C; and
X2=X4=N;
or(iv) X1=X2=C;
X3=X4=N;
R4=halogen or --S(CH2)nR with n being an integer between 1-6 and R is lower alkoxy, alkylthio, phenoxy, phenylthio, unsubstituted or substituted phenyl, --C═
C--R'"'"' wherein R'"'"' is unsubstituted or mono-, di- or trisubstituted phenyl;
R9=R10=H; and
R12=R14=acyloxy;
or(v) R4=NH2 or OH;
R5 is absent;
R9 is --COOH, --CONH2, --C(S)NH2, --C(NH)NH2, or --C(N--NH2)NH2;
X1=X2=X3=C; and
X4=N.
0 Assignments
0 Petitions
Accused Products
Abstract
Structural analogs of the six non-fluorescent N-nucleosides commonly found in RNA and DNA, which are inherently fluorescent under physiological conditions, are identified and methods for their preparation provided. Such analogs may be incorporated into DNA and/or RNA oligonucleotides via either enzymatic or chemical synthesis to produce fluorescent oligonucleotides having prescribed sequences. Such analogous sequences may be identical to, or the analogous complement of, template or target DNA or RNA sequences to which the fluorescent oligonucleotides can be hybridized. Methods of preparing either RNA or DNA oligonucleotide probes of the invention, intermediates used in such methods, and methods of using the probes of the invention in oligonucleotide amplication, detection, identification, and/or hybridization assays are also provided.
-
Citations
4 Claims
-
1. A fluorescent nucleoside, or structural analog thereof, having either of the following structures:
- ##STR2## wherein X1, X2, X3, X4, X5, and X6 =N, O, C, S, or Si, wherein at least one of X1, X2, X3, X4, X5, or X6 =N;
R4 is a reactive group derivatizable with a detectable label wherein said reactive group is selected from the group consisting of NH2, SH, ═
O, and optionally, a linking moiety selected from the group consisting of an amide, a thioether, a disulfide, a combination of an amide a thioether or a disulfide, R1 --(CH2)x --R2 and R1 -R2 --(CH2)x --R3 wherein x is an integer from 1 to 25 inclusive, and R1, R2, and R3 are H, OH, alkyl, acyl, amide, thioether, or disulfide, and wherein said detectable label is selected from the group consisting of radioisotopes, fluorescent or chemiluminescent reporter molecules, antibodies, haptens, biotin, photobiotin, digoxigenin, fluorescent aliphatic amino groups, avidin, enzymes, and acridinium;R5 is H or part of an etheno linkage with R4 ; R6 is H, NH2, SH, or ═
O;R8 and R9 are hydrogen, methyl, bromine, fluorine, or iodine;
alkyl or aromatic substituents, or an optional linking moiety selected from the group consisting of an amide, a thioether, a disulfide linkage, and a combination thereof;R10 is hydrogen, an acid-sensitive/base-stable blocking group, or a phosphorous derivative; R11 =R13 =H; R12 is hydrogen, OH, 3'"'"' amino, 3'"'"'-azido, 3'"'"'-thiol, 3'"'"'-unsaturated or a 3'"'"'-phosphorous derivative; and R14 is H, OH, or OR3 where R3 is a reactive group, protecting group, or additional fluorophore; provided that excluded from such compound is any purine-like compound in which; (i) X1=X4=C;
X2=X3=N;
R4=NH2;
R5=R8 which is absent;
R6=H;
R9 is H or is absent;
R10=H; and
R12=R14=OH;
or(ii) X1=C;
X2=X3=X4=N;
R4=NH2 or H;
R5=R8 which is absent;
R6=NH2;
R9 is H or is absent;
R10=H; and
R12=R14=OH;
or(iii) R4 and R5 in combination form an etheno linkage;
R6=R8=H;
R9 is absent;
X1=X3=C; and
X2=X4=N;
or(iv) X1=X2=C;
X3=X4=N;
R4=halogen or --S(CH2)nR with n being an integer between 1-6 and R is lower alkoxy, alkylthio, phenoxy, phenylthio, unsubstituted or substituted phenyl, --C═
C--R'"'"' wherein R'"'"' is unsubstituted or mono-, di- or trisubstituted phenyl;
R9=R10=H; and
R12=R14=acyloxy;
or(v) R4=NH2 or OH;
R5 is absent;
R9 is --COOH, --CONH2, --C(S)NH2, --C(NH)NH2, or --C(N--NH2)NH2;
X1=X2=X3=C; and
X4=N.
- ##STR2## wherein X1, X2, X3, X4, X5, and X6 =N, O, C, S, or Si, wherein at least one of X1, X2, X3, X4, X5, or X6 =N;
-
2. A polynucleotide probe for the detection or amplification of a target polynucleotide sequence, said probe comprising a fluorescent nucleoside having either of the following structures:
- ##STR3## wherein X1, X2, X3, X4, X5, and X6 =N, O, C, S, or Si, wherein at least one of X1, X2, X3, X4, X5, or X6 =N;
R4 is a reactive group derivatizable with a detectable label wherein said reactive group is selected from the group consisting of NH2, SH, ═
O, and optionally, a linking moiety selected from the group consisting of an amide, a thioether, a disulfide, a combination of an amide a thioether or a disulfide, R1 --(CH2)x --R2 and R1 -R2 --(CH2)x --R3 wherein x is an integer from 1 to 25 inclusive, and R1, R2, and R3 are H, OH, alkyl, acyl, amide, thioether, or disulfide, and wherein said detectable label is selected from the group consisting of radioisotopes, fluorescent or chemiluminescent reporter molecules, antibodies, haptens, biotin, photobiotin, digoxigenin, fluorescent aliphatic amino groups, avidin, enzymes, and acridinium;R5 is H or part of an etheno linkage with R4 ; R6 is H, NH2, SH, or ═
O;R8 and R9 are hydrogen, methyl, bromine, fluorine, or iodine;
alkyl or aromatic substituents, or an optional linking moiety selected from the group consisting of an amide, a thioether, a disulfide linkage, and a combination thereof;R10 is hydrogen, an acid-sensitive/base-stable blocking group, or a phosphorous derivative; R11 =R13 =H; R12 is hydrogen, OH, 3'"'"' amino, 3'"'"'-azido, 3'"'"'-thiol, 3'"'"'-unsaturated or a 3'"'"'-phosphorous derivative; and R14 is H, OH, or OR3 where R3 is a reactive group, protecting group, or additional fluorophore; provided that excluded from such compound is any purine-like compound in which; (i) X1=X4=C;
X2=X3=N;
R4=NH2;
R5=R8 which is absent;
R6=H;
R9 is H or is absent;
R10=H; and
R12=R14=OH;
or(ii) X1=C;
X2=X3=X4=N;
R4=NH2 or H;
R5=R8 which is absent;
R6=NH2;
R9 is H or is absent;
R10=H; and
R12=R14=OH;
or(iii) R4 and R5 in combination form an etheno linkage;
R6=R8=H;
R9 is absent;
X1=X3=C; and
X2=X4=N;
or(iv) X1=X2=C;
X3=X4=N;
R4=halogen or --S(CH2)nR with n being an integer between 1-6 and R is lower alkoxy, alkylthio, phenoxy, phenylthio, unsubstituted or substituted phenyl, --C═
C--R'"'"' wherein R'"'"' is unsubstituted or mono-, di- or trisubstituted phenyl;
R9=R10=H; and
R12=R14=acyloxy;
or(v) R4=NH2 or OH;
R5 is absent;
R9 is --COOH, --CONH2, --C(S)NH2, --C(NH)NH2, or --C(N--NH2)NH2;
X1=X2=X3=C; and
X4=N.
- ##STR3## wherein X1, X2, X3, X4, X5, and X6 =N, O, C, S, or Si, wherein at least one of X1, X2, X3, X4, X5, or X6 =N;
-
3. A method for detecting a target polynucleotide sequence, said method comprising contacting a sample suspected of having said target sequence with an effective amount of a composition comprising a probe in which, as a substitute for any of the six commonly occurring non-fluorescent N-nucleotides, there is provided a fluorescent nucleotide, under conditions which permit hybridization;
- and detecting any hybridization between said probe and said target sequence by observing fluorescence or changes in fluorescence, wherein said fluorescent nucleotide has a structure represented by either of the following structures;
##STR4## wherein X1, X2, X3, X4, X5, and X6 =N, O, C, S, or Si, wherein at least one of X1, X2, X3, X4, X5, or X6 =N;R4 is a reactive group derivatizable with a detectable label wherein said reactive group is selected from the group consisting of NH2, SH, ═
O, and optionally, a linking moiety selected from the group consisting of an amide, a thioether, a disulfide, a combination of an amide a thioether or a disulfide, R1 --(CH2)x --R2 and R1 -R2 --(CH2)x --R3 wherein x is an integer from 1 to 25 inclusive, and R1, R2, and R3 are H, OH, alkyl, acyl, amide, thioether, or disulfide, and wherein said detectable label is selected from the group consisting of radioisotopes, fluorescent or chemiluminescent reporter molecules, antibodies, haptens, biotin, photobiotin, digoxigenin, fluorescent aliphatic amino groups, avidin, enzymes, and acridinium;R5 is H or part of an etheno linkage with R4 ; R6 is H, NH2, SH, or ═
O;R8 and R9 are hydrogen, methyl, bromine, fluorine, or iodine;
alkyl or aromatic substituents, or an optional linking moiety selected from the group consisting of an amide, a thioether, a disulfide linkage, and a combination thereof;R10 is hydrogen, an acid-sensitive/base-stable blocking group, or a phosphorous derivative; R11 =R13 =H; R12 is hydrogen, OH, 3'"'"' amino, 3'"'"'-azido, 3'"'"'-thiol, 3'"'"'-unsaturated or a 3'"'"'-phosphorous derivative; and R14 is H, OH, or OR3 where R3 is a reactive group, protecting group, or additional fluorophore; provided that excluded from such compound is any purine-like compound in which; (i) X1=X4=C;
X2=X3=N;
R4=NH2;
R5=R8 which is absent;
R6=H;
R9 is H or is absent;
R10=H; and
R12=R14=OH;
or(ii) X1=C;
X2=X3=X4=N;
R4=NH2 or H;
R5=R8 which is absent;
R6=NH2;
R9 is H or is absent;
R10=H; and
R12=R14=OH;
or(iii) R4and R5 in combination form an etheno linkage;
R6=R8=H;
R9 is absent;
X1=X3=C; and
X2=X4=N;
or(iv) X1=X2=C;
X3=X4=N;
R4=halogen or --S(CH2)nR with n being an integer between 1-6 and R is lower alkoxy, alkylthio, phenoxy, phenylthio, unsubstituted or substituted phenyl, --C═
C--R'"'"' wherein R'"'"' is unsubstituted or mono-, di- or trisubstituted phenyl;
R9=R10=H; and
R12=R14=acyloxy;
or(v) R4=NH2 or OH;
R5 is absent;
R9 is --COOH, --CONH2, --C(S)NH2, --C(NH)NH2, or --C(N--NH2)NH2;
X1=X2=X3=C; and
X4=N. - View Dependent Claims (4)
- and detecting any hybridization between said probe and said target sequence by observing fluorescence or changes in fluorescence, wherein said fluorescent nucleotide has a structure represented by either of the following structures;
Specification