Biochemical sensor device and method

• US 5,653,862 A
• Filed: 04/15/1996
• Issued: 08/05/1997
• Est. Priority Date: 04/15/1996
• Status: Expired due to Fees

• Current Assignee: Dade Behring, Inc. (Siemens AG)
• Original Assignee: DADE Chemistry Systems Incorporated (Siemens AG)
• Inventors: Parris, Norman Alfred
• Primary Examiner(s): Gorgos, Kathryn L.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US08/632,202
• Time in Patent Office: 477 Days
• Field of Search: 435/817, 435/12, 435/14, 435/25, 204/403, 204/409, 205/777.5
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence

Abstract

Degradation of multielectrochemical sensors in a common flow stream is averted by positioning any sensors having as its associated enzyme an oxidoreductase enzyme capable of producing, as a result interaction with its respective substrate, an oxidizing agent downstream of the other enzyme sensors not having such oxidoreductase enzyme.

Claims

1. A bioanalytical measurement device for sensing at least two components of a liquid sample, the device comprising:

a housing having first and second biochemical sensors, each having an enzyme associated therewith, the housing also having a single flow channel to flow liquid samples over the first and second biochemical sensors in a single direction; and

,

measurement means connected with the first and second biochemical sensors,

the first biochemical sensor having an oxidase enzyme selected from the group consisting of lactate oxidase, lactose oxidase, and glucose oxidase that produces an oxidizing agent as a result of interaction with its substrate, at least some of oxidizing agent being released into the flow channel,

the first biochemical sensor positioned in the flow channel downstream of the second biochemical sensor so that oxidizing agents released into the flow channel by the first biochemical sensor do not adversely affect the sensing of the second biochemical sensor.

2. The device of claim 1 wherein the oxidizing agent is hydrogen peroxide.

3. A method for sensing at least two components of a liquid sample using first and second biochemical sensors having enzymes associated therewith, the first biochemical sensor having an oxidase enzyme selected from the group consisting of lactase oxidase, lactose oxidase, and glucose oxidase that produces and oxidizing agent as a result of interaction with its substrate, the method comprising:

flowing liquid samples within a flow channel over the first and second biochemical sensors in a single direction;

positioning the first biochemical sensor device in the flow channel downstream of the second biochemical sensor so that oxidizing agents released into the flow channel by the first biochemical sensor do not adversely affect the sensing of the second biochemical sensor; and

,

measuring the chemical activity of each biochemical sensor.

4. The method of claim 3 wherein the oxidizing agent is hydrogen peroxide.