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Detecting or quantifying multiple analytes using labelling techniques

  • US 5,656,207 A
  • Filed: 05/11/1995
  • Issued: 08/12/1997
  • Est. Priority Date: 06/24/1989
  • Status: Expired due to Term
First Claim
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1. A specific binding assay for two or more individual analytes in a liquid test sample which assay comprises:

  • a) contacting the sample with two or more specific labeled reagents, wherein each such reagent comprisesi) a member of a specific binding pair to which an individual analyte is a member, andii) a different, specific light-emitting label covalently or non-covalently joined to said member, in order to form at least one labeled said specific binding pair,b) physically or chemically differentiating each said labeled specific binding pair from said labeled reagents,c) generating and measuring light emission from each specifically labeled specific binding pair, as a measure of the presence or amount of each said analyte,wherein each of the labels emit light at distinguishable emission maxima, or emit peak energy at distinguishable times after the generation of said emission, from at least one other of said labels.

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