Detecting or quantifying multiple analytes using labelling techniques
First Claim
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1. A specific binding assay for two or more individual analytes in a liquid test sample which assay comprises:
- a) contacting the sample with two or more specific labeled reagents, wherein each such reagent comprisesi) a member of a specific binding pair to which an individual analyte is a member, andii) a different, specific light-emitting label covalently or non-covalently joined to said member, in order to form at least one labeled said specific binding pair,b) physically or chemically differentiating each said labeled specific binding pair from said labeled reagents,c) generating and measuring light emission from each specifically labeled specific binding pair, as a measure of the presence or amount of each said analyte,wherein each of the labels emit light at distinguishable emission maxima, or emit peak energy at distinguishable times after the generation of said emission, from at least one other of said labels.
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Abstract
A method for the assay, detection etc. of each of a plurality of substances of interest in a sample comprises labelling each of the substances with one or more components each capable of taking part in a respective distinguishable chemiluminescent reaction. Luminescent reagents for use in the method are also disclosed.
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Citations
29 Claims
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1. A specific binding assay for two or more individual analytes in a liquid test sample which assay comprises:
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a) contacting the sample with two or more specific labeled reagents, wherein each such reagent comprises i) a member of a specific binding pair to which an individual analyte is a member, and ii) a different, specific light-emitting label covalently or non-covalently joined to said member, in order to form at least one labeled said specific binding pair, b) physically or chemically differentiating each said labeled specific binding pair from said labeled reagents, c) generating and measuring light emission from each specifically labeled specific binding pair, as a measure of the presence or amount of each said analyte, wherein each of the labels emit light at distinguishable emission maxima, or emit peak energy at distinguishable times after the generation of said emission, from at least one other of said labels. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A kit for the detection of more than one analyte in a test sample comprising two or more specifically labeled reagents, each said reagent comprising
(i) a member of a specific binding pair to which an individual analyte is a member, and (ii) a different, specific label covalently or non-covalently joined to said binding pair member, said label being detectable by the emission of light from said label, wherein each of the labels emit light at separate and distinct emission maxima, or emit peak energy at separate and distinct times after the generation of said emission, from at least one other of said labels, and wherein upon contacting said reagents with a test sample containing said analytes, the presence or amount of each said analyte is determined after the initiation of light emission.
Specification