Homogeneous bioassay using acoustic emission spectroscopy
First Claim
1. A system for homogeneous bioassay using acoustic energy comprising:
- a measurement cell containing a binding reaction of mobile biorecognition molecules, said binding reaction selected from the group consisting of ligand/receptor binding reactions and complementary nucleic acid binding reactions, said complementary nucleic acid binding reactions selected from the group consisting of DNA/DNA, DNA/RNA, RNA/RAN binding reactions, PCR reactions and DNA/protein binding reactions;
a passive acoustic energy transducer coupled to the measurement cell for detecting acoustic energy emissions generated by a binding reaction of mobile biorecognition molecules and generating a corresponding analog electrical signal, said passive acoustic energy transducer comprising a wideband frequency response transducer responsive to a specified broad frequency spectrum of acoustic energy and constructed for peak resonance at an odd multiple 1/2 the wavelength of the center frequency of the specified broad frequency spectrum;
a signal conditioner for filtering and amplifying said electrical signal to provide a conditioned electrical signal;
and a signal analyzer for determining spectra of the conditioned electrical signal corresponding to characteristic acoustic energy emissions of the binding reaction of mobile biorecognition molecules.
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Abstract
A system and method for homogeneous bioassay uses acoustic energy emissions. A measurement cell is designed for binding reactions of mobile biorecognition molecules. The binding reaction contained in the measurement cell is for example a ligand/receptor binding reaction or a complementary nucleic acid binding reaction. A passive acoustic energy transducer is coupled to the measurement cell for detecting acoustic energy generated by the binding reaction of mobile biorecognition molecules. The passive acoustic energy transducer generates a corresponding analog electrical signal. A signal conditioner coupled to the passive acoustic energy transducer filters and amplifies the electrical signal. A signal analyzer determines spectra of the conditioned electrical signal corresponding to characteristic acoustic energy emissions of the binding reaction of mobile biorecognition molecules. The transducer and signal analyzer may be constructed for spectral analysis of a broad band frequency spectrum in the frequency domain or spectral waveform analysis of a narrow band amplitude spectrum in the time domain. The homogeneous bioassay is applicable for example to ligand/receptor binding reactions such as antigen/antibody binding reactions and enzyme/substrate binding reactions and to complementary nucleic acid binding reactions such as DNA/DNA and RNA/DNA binding reactions.
54 Citations
38 Claims
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1. A system for homogeneous bioassay using acoustic energy comprising:
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a measurement cell containing a binding reaction of mobile biorecognition molecules, said binding reaction selected from the group consisting of ligand/receptor binding reactions and complementary nucleic acid binding reactions, said complementary nucleic acid binding reactions selected from the group consisting of DNA/DNA, DNA/RNA, RNA/RAN binding reactions, PCR reactions and DNA/protein binding reactions; a passive acoustic energy transducer coupled to the measurement cell for detecting acoustic energy emissions generated by a binding reaction of mobile biorecognition molecules and generating a corresponding analog electrical signal, said passive acoustic energy transducer comprising a wideband frequency response transducer responsive to a specified broad frequency spectrum of acoustic energy and constructed for peak resonance at an odd multiple 1/2 the wavelength of the center frequency of the specified broad frequency spectrum; a signal conditioner for filtering and amplifying said electrical signal to provide a conditioned electrical signal; and a signal analyzer for determining spectra of the conditioned electrical signal corresponding to characteristic acoustic energy emissions of the binding reaction of mobile biorecognition molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A system for homogeneous bioassay using acoustic energy comprising:
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a measurement cell for containing a binding reaction of mobile biorecognition molecules, said binding reaction selected from the group consisting of ligand/receptor binding reactions and complementary nucleic acid binding reactions, said complementary nucleic acid binding reactions selected from the group consisting of DNA/DNA, DNA/RNA, RNA/RNA binding reactions, PCR reactions and DNA/protein binding reactions; a passive acoustic energy transducer coupled to the measurement cell for detecting acoustic energy emissions generated by a binding reaction of mobile biorecognition molecules and generating a corresponding analog electrical signal, and wherein the acoustic energy transducer comprises a plurality of transducer plates responsive to different frequency bands or channels of acoustic energy for differentiating by channel the characteristic acoustic energy generated by the binding reaction; a signal conditioner for filtering and amplifying said electrical signal to provide a conditioned electrical signal; and a signal analyzer for determining spectra of the conditioned electrical signal corresponding to characteristic acoustic energy emissions of the binding reaction of mobile biorecognition molecules. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A method for homogeneous bioassay for an unknown analyte, said method comprising:
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reacting in a measurement cell mobile biorecognition molecules in a binding reaction selected from the group consisting of ligand/receptor binding reactions and complementary nucleic acid binding reactions, said complementary nucleic acid binding reactions selected from the group consisting of DNA/DNA reactions, RNA/DNA reactions, RNA/RNA reactions, peptide nucleic acid/DNA reactions, PCR reactions and DNA/protein reactions, wherein said biorecognition molecules correspond to the unknown analyte; detecting acoustic energy emissions generated by the binding reaction of mobile recognition molecules with a plurality of passive acoustic energy transducer plates responsive to different frequency bands or channels of acoustic energy for differentiating by channel the characteristic acoustic energy generated by the binding reaction and generating a corresponding analog electrical signal; filtering and amplifying said electric signal; and analyzing spectra of the electrical signal corresponding to characteristic acoustic energy emissions of the binding reaction of mobile biorecognition molecules to identify the unknown analyte. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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Specification