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Method of preventing undesired binding in solid phase assays

  • US 5,656,504 A
  • Filed: 04/25/1995
  • Issued: 08/12/1997
  • Est. Priority Date: 10/26/1992
  • Status: Expired due to Term
First Claim
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1. A specific binding assay method for determining an analyte in a sample with reduced nonspecific interference from non-analyte constituents in said sample comprising:

  • (a) providing a solid phase comprising a polymer surface having immobilized thereto a ligand capable of specifically binding said analyte;

    (b) adding one or more monomeric, oligomeric or polymeric chain fragments of said polymer to said sample wherein said fragments are at least partially soluble in said sample, to form a reaction medium;

    (c) contacting said reaction medium with said solid phase; and

    (d) measuring any binding of said analyte to said contacted solid phase to determine the presence or amount of said analyte in said sample, wherein said nonspecific interference is reduced in that said non-analyte constituents bind to said fragments in said reaction medium rather than to said solid phase.

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