Nucleic acid-amplified immunoassay probes

• US 5,656,731 A
• Filed: 07/01/1993
• Issued: 08/12/1997
• Est. Priority Date: 10/15/1987
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Chiron Corporation (Novartis Ag)
• Inventors: Urdea, Michael S.
• Primary Examiner(s): Elliott, George C.
• Assistant Examiner(s): Marschel, Ardin H.
• Application Number:
  US08/085,681
• Time in Patent Office: 1,503 Days
• Field of Search: 435/7.1, 435/6, 435/91, 435/810, 435/7.7, 435/91.1, 436/501, 536/22.1, 536/23.1, 536/24.31-24.33, 536/24.1, 935/77, 935/78, 530/387.1, 530/391.1, 530/391.3, 530/391.5
• US Class Current: 530/391.1
• CPC Class Codes: C07H 19/06 Pyrimidine radicals
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6804 Nucleic acid analysis using immunogens immunoassay G01N33/53
• CPC Class Codes: C12Q 1/682 Signal amplification
• CPC Class Codes: C12Q 2525/301 Hairpin oligonucleotides
• CPC Class Codes: C12Q 2525/313 Branched oligonucleotides
• CPC Class Codes: C12Q 2531/143 Promoter based amplification, e.g. NASBA, 3SR, TAS
• CPC Class Codes: C12Q 2563/179 the label being a nucleic acid
• CPC Class Codes: G01N 2333/045 Cytomegalovirus
• CPC Class Codes: G01N 33/532 Production of labelled immunochemicals
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

In one aspect of this invention, various protein/nucleic acid hybrid probes are described which can be used to amplify the detectable signal in immunoassays. The protein moiety is capable of functioning either as an antibody or an antigen. The nucleic acid moiety serves as a signal amplifier. In another aspect, various methods of amplifying the detectable signal in immunoassays by use of the hybrid probes and related polynucleotide probes are disclosed.

Claims

1. A molecular probe for use as a signal amplifier in immunoassays comprising:

(a) a first domain (A) which is a polypeptide and functions as an antibody specific for a known antigen;

(b) a second domain (B) which is a double-stranded polynucleotide capable of functioning as a promoter for a DNA-dependent RNA polymerase enzyme activity; and

(c) a third domain (C) which is either a single- or double-stranded polynucleotide with a random sequence, whose transcript is incapable of being translated into a polypeptide, and is directly 3'"'"' to the second domain, such that the third domain is a template for the promoter activity of the second domain, wherein said first domain is covalently conjugated to said second domain and said second domain is covalently conjugated to said third domain.

2. The probe of claim 1 in which the DNA-dependent RNA polymerase activity is derived from the bacteriophage T7.

3. The probe of claim 1 in which the DNA-dependent RNA polymerase activity is derived from the bacteriophage T3.

4. The probe of claim 1 in which the DNA-dependent RNA polymerase activity is derived from the bacteriophage SP6.

5. The probe of claim 1 in which the second domain B is at least 10 and no more than 40 nucleotides long.

6. The probe of claim 1 in which the second domain B is at least 20 and no more than 35 nucleotides long.

7. The probe of claim 5 in which the third domain C is at least 30 and no more than 80 nucleotides long.

8. The probe of claim 5 in which the third domain C is at least 35 and no more than 50 nucleotides long.

9. The probe of claim 2 in which the sequence for the second domain B comprises the sequence:

space="preserve" listing-type="equation">5'"'"'-TAA TAC GAC TCA CTA TA-3'"'"'

space="preserve" listing-type="equation">3'"'"'-ATT ATG CTG AGT GAT AT-5'"'"'

10. The probe of claim 2 in which the nucleotide sequence of the second domain B is:

space="preserve" listing-type="equation">5'"'"'-CTG GCT TAT CGA AAT TAA TAC GAC TCA CTA TA-3'"'"'

space="preserve" listing-type="equation">3'"'"'-GAC CGA ATA GCT TTA ATT ATG CTG AGT GAT AT-5'"'"'

11. The probe of claim 1 in which the 3'"'"' end of the template is adjacent to the second domain B, and is a cytosine residue.

12. The probe of claim 1 in which the sequence for the third domain C is:

space="preserve" listing-type="equation">5'"'"'-GGG AGA TGT GGT TGT CGT ACT TAG CGA AAT ACT GTC CGA GTC G-3'"'"'

space="preserve" listing-type="equation">3'"'"'-CCC TCT ACA CCA ACA GCA TGA ATC GCT TTA TGA CAG GCT CAG C-5'"'"'

13. The probe of claim 1 in which the transcript of the third domain C has two subdomains:

(a) a first subdomain, c₁, which is capable of hybridizing to an oligonucleotide capture linker, the capture linker being capable of hybridizing to a polynucleotide immobilized on a solid substrate; and

(b) a second subdomain, c₂, which is capable of binding to an oligonucleotide label linker, the label linker capable of binding to a quantifiable probe.

14. The probe of claim 1 in which a functional unit comprising the second and third domains, B and C, are present in multiple repeating units.

15. A molecular probe for use as a signal amplifier in immunoassays comprising:

(a) a first domain (A) comprising a polypeptide capable of functioning as an antibody which binds specifically to a known analyte; and

(b) a second domain (M) comprising a multiplicity of single-stranded oligonucleotide subunits that are capable of hybridizing specifically to a single-stranded nucleic acid of interest, wherein said second domain (M) consists of a branched polynucleotide having a minimum of three termini, which are of the 3'"'"' or 5'"'"' type, and at least one covalent branch point, wherein the multiplicity of single-stranded oligonucleotide subunits are bonded directly or indirectly only via covalent bonds and wherein said first domain is covalently conjugated to said second domain.

16. The probe of claim 15 in which each oligonucleotide unit of the second domain M, comprises about 15 to 50 bases and has a GC content in the range of about 40% to about 60%.

17. The probe of claim 15 in which the probe is a comb-like multimer comprising an oligonucleotide backbone with one or more pendant chains composes of one or more oligonucleotide units.

18. The probe of claim 15 in which the first domain is conjugated to the second domain via a cross-linking agent.

19. The probe of claim 18 in which the cross-linking agent is covalently bonded to the oligonucleotide via the N⁴ -position of N⁴ -modified cytosine bases.

20. The probe of claim 15 wherein the second domain, M, contains one or more modified nucleotides.

21. The probe of claim 15 wherein the oligonucleotide subunits of the second domain are linked directly to each other through phosphodiester bonds.

22. The probe of claim 15 wherein the oligonucleotide subunits of the second domain are linked to each other via a cross-linking agent.

23. The probe of claim 22 wherein the cross-linking agent is covalently bonded to the oligonucleotide subunits of the second domain via the N⁴ -position of N⁴ -modified cytosine bases.

24. The probe of claim 15 wherein at least a portion of the oligonucleotide subunits of the second domain are linked via a multifunctional moiety derived from a compound of the formula:

##STR10## where R is an organic moiety, preferably a nucleic acid, R¹ is a hydroxyl protecting group that can be removed under conditions that do not remove synthetic nucleic acid from a solid phase and do not remove exocyclic nitrogen or phosphate protecting groups, X is a phosphorus-containing group that facilitates nucleic acid synthesis, Y is a radical derived from a nucleophilic group, and R² is R¹ or a blocking or protective group that can be removed and replaced with hydrogen without affecting R¹.

25. The probe of claim 15 wherein at least a portion of the oligonucleotide subunits are linked via a multifunctional moiety derived from a compound of the formula:

##STR11## where Z is a nucleophile, R¹ is a protective group that is generally base-stable and acid sensitive, R² is hydrogen or methyl, R³ is a protective group that can be removed and replaced with hydrogen without affecting R¹, R⁵ is a phosphorus derivative that enables addition of nucleotides to the 5'"'"' position of an oligonucleotide chain during chemical synthesis, R⁶ is methyl, hydrogen, I, Br, or F, and X is an integer in the range of 1 to 8, inclusive.

26. The probe of claim 15 wherein at least a portion of the oligonucleotide subunits are linked via a multifunctional moiety derived from a compound of the formula:

##STR12## where R is a hydroxyl protecting group, iPr is isopropyl, and R¹ is methyl or betacyanoethyl.

27. The probe of claim 15 in which the second domain (M) has at least one covalent branch point comprising a cytosine base which has been modified at the N⁴ position, wherein each of said modified cytosine bases has at least one of said single-stranded oligonucleotide subunits covalently bonded directly or indirectly via said N⁴ position.

28. A molecular probe for use as a signal amplifier in immunoassays comprising:

(a) a first domain (A) comprising a polypeptide capable of functioning as an antibody which binds specifically to a known analyte; and

(b) a second domain (M) comprising a multiplicity of single-stranded oligonucleotide subunits that are capable of hybridizing specifically to a single-stranded nucleic acid of interest, wherein said multiplicity of subunits comprises at least two identical subunits, wherein said second domain (M) consists of a branched polynucleotide having a minimum of three termini, which are of the 3'"'"' or 5'"'"' type, and at least one covalent branch point, wherein the multiplicity of single-stranded oligonucleotide subunits are bonded directly or indirectly only via covalent bonds and wherein said first domain is covalently conjugated to said second domain.

29. The probe of claim 28 in which the second domain (M) has at least one covalent branch point comprising a cytosine base which has been modified at the N⁴ position, wherein each of said modified cytosine bases has at least one of said single-stranded oligonucleotide subunits covalently bonded directly or indirectly via said N⁴ position.

30. A molecular probe for use as a signal amplifier in immunoassays comprising:

(a) a first domain (A) comprising a polypeptide capable of functioning as an antibody which binds specifically to a known analyte; and

(b) a second domain (M) comprising a multiplicity of single-stranded DNA oligonucleotide subunits that are capable of hybridizing specifically to a single-stranded nucleic acid of interest, wherein said second domain (M) consists of a branched polynucleotide having a minimum of three termini, which are of the 3'"'"' or 5'"'"' type, and at least one covalent branch point, wherein the multiplicity of single-stranded oligonucleotide subunits are bonded directly or indirectly only via covalent bonds and wherein said first domain is covalently conjugated to said second domain.

31. The probe of claim 30 in which the second domain (M) has at least one covalent branch point comprising a cytosine base which has been modified at the N⁴ position, wherein each of said modified cytosine bases has at least one of said single-stranded oligonucleotide subunits covalently bonded directly or indirectly via said N⁴ position.