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Nucleic acid-amplified immunoassay probes

  • US 5,656,731 A
  • Filed: 07/01/1993
  • Issued: 08/12/1997
  • Est. Priority Date: 10/15/1987
  • Status: Expired due to Term
First Claim
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1. A molecular probe for use as a signal amplifier in immunoassays comprising:

  • (a) a first domain (A) which is a polypeptide and functions as an antibody specific for a known antigen;

    (b) a second domain (B) which is a double-stranded polynucleotide capable of functioning as a promoter for a DNA-dependent RNA polymerase enzyme activity; and

    (c) a third domain (C) which is either a single- or double-stranded polynucleotide with a random sequence, whose transcript is incapable of being translated into a polypeptide, and is directly 3'"'"' to the second domain, such that the third domain is a template for the promoter activity of the second domain, wherein said first domain is covalently conjugated to said second domain and said second domain is covalently conjugated to said third domain.

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