Biocompatible perforated membranes, processes for their preparation, their use as a support in the in vitro growth of epithelial cells, the artificial skin obtained in this manner, and its use in skin grafts

• US 5,658,331 A
• Filed: 06/07/1995
• Issued: 08/19/1997
• Est. Priority Date: 06/01/1990
• Status: Expired due to Term

• Current Assignee: Fidia S.P.A.
• Original Assignee: Fidia S.P.A.
• Inventors: Della Valle, Francesco, Romeo, Aurelio, Calderini, Gabriella, Rastrelli, Alessandro
• Primary Examiner(s): ISABELLA, DAVID J
• Application Number:
  US08/478,541
• Time in Patent Office: 804 Days
• Field of Search: 623/1, 623/2, 623/8, 623/11, 623/12, 623/15, 623/16, 623/66, 424/422, 424/425, 424/447, 424/448
• US Class Current: 128/898
• CPC Class Codes: A61F 2/105 Skin implants, e.g. artificial skin
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61L 27/60 Materials for use in artificial skin
• CPC Class Codes: C12N 2533/70 Polysaccharides
• CPC Class Codes: C12N 2533/80 Hyaluronan
• CPC Class Codes: C12N 2539/10 Coating allowing for selective detachment of cells, e.g. thermoreactive coating
• CPC Class Codes: C12N 5/0062 General methods for three-dimensional culture
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0629 Keratinocytes; Whole skin
• CPC Class Codes: Y10T 428/24273 including aperture

Abstract

The invention relates to biocompatible membranes constructed of materials of natural, synthetic or semisynthetic origin, and having a thickness of between 10 and 500μ, characterized by containing an ordered series of holes of a constant size between 10 and 1000μ, separated from each other by a constant distance of between 50 and 1000μ, and obtained by perforation by mechanical, thermal laser or ultraviolet radiation means, they being suitable for use as a support for the in vitro growth of epithelial cells, the invention also relating to the artificial skin obtained thereby and its use in grafts.

Claims

1. A therapeutic method for treatment of lesions of the body surface which comprises transplantation on said lesions of an artificial skin composed of a biocompatible support membrane consisting of materials of natural, synthetic or semisynthetic origin, and having a thickness of between 10 and 500 μ

m, which contains an ordered series of holes of a defined and constant size between 10 and 1000 μ

m, separated from each other by a constant distance of between 50 and 1000 μ

m, together with autologous or heterologous epithelial cells in the active proliferation stage present within said holes and on both sides of said membrane.

2. The therapeutic method as claimed in claim 1, in which the epithelial cells are keratinocyte microcolonies.

3. The therapeutic method as claimed in claim 1, comprising shaping said artificial skin on the basis of the area to be treated and suturing it to said area.

4. The therapeutic method as claimed in claim 1, in which the holes size is between 40 and 70 μ

m.

5. The therapeutic method as claimed in claim 1, in which the distance between holes is 80 μ

m.

6. The therapeutic method as claimed in claim 1, in which membranes have a thickness comprised between 20 and 40 μ

m.

7. The therapeutic method as claimed in claim 1, in which the biocompatible material of natural origin is selected from the group consisting of collagen, coprecipitates of collagen and glycosaminoglycans, cellulose, gelled polysaccharides, and natural rubbers, either alone or in mixture with each other or with polymers of synthetic or semisynthetic origin, in the presence of precipitating or gelling agents.

8. The therapeutic method as claimed in claim 7 in which the gelled polysaccharides are selected from the group consisting of chitin, chitosan, pectins or pectic acids, agar, agarose, xanthan gum, gellano, alginic acid or alginates, polymannans or polyglucans and starches.

9. The therapeutic method as claimed in claim 1, in which the biocompatible material of synthetic origin is chosen from the group consisting of polylactic acid, polyglycolic acid or copolymers thereof or their derivatives, polydioxanones, polyphosphazenes, polysulphones and polyurethanes.

10. The therapeutic method as claimed in claim 1, in which the biocompatible material of semisynthetic origin is chosen from the group consisting of semisynthetic derivatives of natural polymers crosslinked with crosslinking agents and derivatives of cellulose, of alginic acid, of starch, of hyaluronic acid, of chitin or chitosan, of gellano, of xanthan, of pectins or pectic acids, of polyglucans, of polymannans, of agar, of agarose, of natural rubbers or of glycosaminoglycans.

11. The therapeutic method as claimed in claim 10, in which the semysinthetic derivative of a natural polymer is collagen.

12. The therapeutic method as claimed in claim 10, in which the biocompatible membrane consists of hyaluronic acid benzyl ester with 100 percent of esterification.

13. The therapeutic method as claimed in claim 10, in which the crosslinking agent is selected from the group consisting of dialdehydes or their precursors, bicarboxylic acids or halides thereof and diamines.

14. The therapeutic method as claimed in claim 1, in which the materials of synthetic origin are selected from the group consisting of silicone, silane or siloxane rubbers, fluoropolymers, polystyrene, vinyl polychloride, polyacrylate or derivatives thereof, polyhydroxyacrylate, polyhydroxymethacrylate, carboxyvinyl polymers and their derivatives, maleic anhydride polymers and their derivatives, polyvinylchloride, polyvinylalchol and its derivatives, polyethylene and polypropylene.

15. The therapeutic method as claimed in claim 14, in which fluoropolymers are selected from the group consisting of polyfluoroethylene, polyfluoropropylene and polyfluoroethers.

16. The therapeutic method as claimed in claim 1, wherein the artificial skin is applied to the area to be treated on either of its two sides.

17. The therapeutic method as claimed in claim 1, wherein said artificial skin is cryopreserved.