Biosensor and method for producing the same
First Claim
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1. A method for producing a biosensor comprising the steps of:
- forming a first layer containing an enzyme and a hydrophilic polymer by using water as the medium on a face of an insulating base in close contact with an electrode system including a working electrode and a counter electrode which are provided on said insulating base; and
forming a second layer containing a buffer on said first layer by using an organic solvent solution of a lipid which does not dissolve said hydrophilic polymer.
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Abstract
A biosensor for rapid quantification of a specific component contained in various biological samples with high accuracy has an electrically insulating base, an electrode system including a working electrode and a counter electrode formed on one face of the insulating base, and a reaction layer formed on the insulating base in close contact with the electrode system. The reaction layer contains at least a hydrophilic polymer, a buffer and an enzyme which is separated from the buffer.
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Citations
18 Claims
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1. A method for producing a biosensor comprising the steps of:
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forming a first layer containing an enzyme and a hydrophilic polymer by using water as the medium on a face of an insulating base in close contact with an electrode system including a working electrode and a counter electrode which are provided on said insulating base; and forming a second layer containing a buffer on said first layer by using an organic solvent solution of a lipid which does not dissolve said hydrophilic polymer. - View Dependent Claims (2, 3)
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4. A method for producing a biosensor comprising the steps of:
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forming a first layer containing a buffer and a hydrophilic polymer by using water as the medium on a face of an insulating base in close contact with an electrode system including a working electrode and a counter electrode provided on said insulating base; and forming a second layer containing a hydrophilic polymer and an enzyme on said first layer by using an organic solvent as the medium that does not dissolve said hydrophilic polymer contained in the first layer. - View Dependent Claims (5, 6, 7)
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8. A method for producing a biosensor comprising:
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a first step of spreading an aqueous solution containing a hydrophilic polymer and a buffer on an insulating base in close contact with an electrode system including a working electrode and a counter electrode provided on a face of said insulating base and drying the spread solution, and a second step of spreading an organic solvent solution containing at least an enzyme and a hydrophilic polymer over the layer and drying the spread solution. - View Dependent Claims (9, 10)
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11. A method for producing a biosensor comprising the steps of:
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forming a first layer containing an enzyme and a hydrophilic polymer by using water as the medium on a face of an insulating base in close contact with an electrode system including a working electrode and a counter electrode which are provided on said insulating base; and forming a second layer containing a buffer on said first layer by using an organic solvent solution of a hydrophilic polymer, which solvent does not dissolve said hydrophilic polymer contained in the first layer. - View Dependent Claims (12, 13, 14, 15)
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16. A biosensor comprising:
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an electrical insulating base, an electrode system including a working electrode and a counter electrode which are provided on a face of said insulating base, and a reaction layer formed on said insulating base in close contact with said electrode system;
whereinsaid reaction layer is a laminate of at least two layers, and wherein a first reaction layer is in contact with said electrode system and contains an enzyme, an electron acceptor and a hydrophilic polymer, and a second reaction layer is on top of said first reaction layer and contains a buffer and a lipid, said enzyme being separated from said buffer.
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17. A biosensor comprising,
an electrical insulating base, an electrode system including a working electrode and a counter electrode which are provided on a face of said insulating base, and a reaction layer formed on said insulating base in close contact with said electrode system; - wherein
said reaction layer is a laminate of at least two layers, and wherein a first reaction layer is in contact with said electrode system and contains an enzyme and an electron acceptor and a second reaction layer is on top of said first reaction layer and contains a buffer and a hydrophilic polymer, said enzyme being separated from said buffer.
- wherein
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18. A biosensor comprising,
an electrical insulating base, an electrode system including a working electrode and a counter electrode which are provided on a face of said insulating base and a reaction layer formed on said insulating base in close contact with said electrode system; - wherein
said reaction layer is a laminate of at least three layers, and wherein a first reaction layer is in contact with said electrode system and contains an enzyme and an electron acceptor, a second reaction layer is on top of said first reaction layer and contains a buffer and a hydrophilic polymer and a third reaction layer is on top of said second reaction layer and contains a lipid, said enzyme being separated from said buffer.
- wherein
Specification