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Oligonucleotide population preparation

  • US 5,658,736 A
  • Filed: 01/16/1996
  • Issued: 08/19/1997
  • Est. Priority Date: 01/16/1996
  • Status: Expired due to Term
First Claim
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1. A method for producing a sequence tag from a polynucleotide, said method comprising:

  • (a) digesting said polynucleotide with a type II restriction enzyme to produce a first cohesive end;

    (b) providing a promoter-linker cassette comprising an RNA polymerase promoter, a restriction site for a type IIs restriction enzyme and a second cohesive end which is complementary to said first cohesive end produced by said type II restriction enzyme;

    (c) ligating the digested polynucleotide to the cassette by joining said first cohesive end and said second cohesive end;

    (d) digesting the ligated product of step (c) with said type IIs restriction enzyme; and

    (e) transcribing the resulting construct from said promoter, thereby producing a sequence tagged polyribonucleotide.

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