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Long lifetime anisotropy (polarization) probes for clinical chemistry, immunoassays, affinity assays and biomedical research

  • US 5,660,991 A
  • Filed: 10/28/1994
  • Issued: 08/26/1997
  • Est. Priority Date: 10/28/1994
  • Status: Expired due to Term
First Claim
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1. A method of conducting an assay of a sample of interest, comprising the steps of:

  • coupling a luminescent asymmetric transition metal-ligand complex to an analyte in the sample of interest to form a coupled analyte, wherein the asymmetric transition metal-ligand complex-coupled analyte is capable of emitting polarized fluorescent light after being excited with linearly polarized electromagnetic light energy;

    exciting the coupled analyte with linearly polarized electromagnetic light energy to cause the coupled analyte to emit polarized fluorescent light; and

    measuring the polarization of the fluorescent light emission as a measure of a biological characteristic of the analyte in the sample of interest.

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