.beta.3 integrin subunit specific polypeptides, cDNAS which encode these polypeptides and methods of producing these polypeptides

• US 5,661,005 A
• Filed: 02/02/1996
• Issued: 08/26/1997
• Est. Priority Date: 11/10/1994
• Status: Expired due to Term

• Current Assignee: The Scripps Research Institute, Trustees Of The University Of Pennsylvania (University of Pennsylvania)
• Original Assignee: The Scripps Research Institute, Trustees Of The University Of Pennsylvania (University of Pennsylvania)
• Inventors: O'Toole, Timothy, Ginsberg, Mark, Shattil, Sanford
• Primary Examiner(s): Ulm, John
• Assistant Examiner(s): BROWN, KAREN
• Application Number:
  US08/597,583
• Time in Patent Office: 571 Days
• Field of Search: 536/23.5, 435/320.1, 435/325, 435/69.1, 435/252.3
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/70557 Integrin beta3-subunit-containing molecules, e.g. CD41, CD51, CD61

Abstract

β₃ integrin subunit specific polypeptides, cDNAs which encode these polypeptides and methods of producing these polypeptides are provided.

Claims

1. A purified and isolated cDNA encoding a human β

₃ integrin subunit specific polypeptide, which polypeptide comprises SEQ ID NO;

2 or 4.

2. The cDNA of claim 1 comprising SEQ ID NO:

1 or 3.

3. An expression vector comprising a DNA sequence encoding a β

₃ integrin subunit specific polypeptide, which polypeptide comprises SEQ ID NO;

2 or 4.

4. The expression vector of claim 3 wherein the DNA sequence comprises SEQ ID NO:

1 or 3.

5. A cell line transfected with the expression vector of claim 3 or claim 4.

6. A method of making a β

₃ integrin subunit specific polypeptide comprising;

culturing a cell transfected by an expression vector comprising DNA encoding a β

₃ integrin subunit specific polypeptide, which polypeptide comprises SEQ ID NO;

2 or 4; and

recovering the β

₃ integrin subunit specific polypeptide from the cell.

7. The method of claim 6 where the DNA comprises SEQ ID NO:

1 or 3.