DNA encoding a human serotonic (5-HT.sub.2) receptor and uses thereof

• US 5,661,024 A
• Filed: 11/30/1994
• Issued: 08/26/1997
• Est. Priority Date: 10/31/1989
• Status: Expired due to Term

• Current Assignee: Lundbeck Research USA, Inc. (Lundbeck Foundation)
• Original Assignee: Synaptic Pharmaceutical Corporation (Lundbeck Foundation)
• Inventors: Branchek, Theresa, Hartig, Paul R., Kao, Hung-Teh
• Primary Examiner(s): BICKEL, MINDY B
• Application Number:
  US08/347,591
• Time in Patent Office: 1,000 Days
• Field of Search: 435/69.1, 435/70.5, 435/71.1, 435/172.1, 435/240.2, 435/252.3, 435/320.1, 435/235.1, 536/23.5, 935/69, 935/70, 935/72, 935/73
• US Class Current: 435/356
• CPC Class Codes: C07K 14/70571 for neuromediators, e.g. serotonin receptor, dopamine receptor

Abstract

The present invention provides an isolated nucleic acid molecule encoding an 5-HT₂ receptor, and an isolated protein which is a human 5-HT₂ receptor.

The invention also provides vectors comprising DNA molecules encoding a human 5-HT₂ receptor, and vectors adapted for expression of the 5-HT₂ receptor in bacterial, yeast, or mammalian cells.

In addition, the invention provides a DNA probe useful for detecting nucleic acid encoding the 5-HT₂ receptor, a method for determining whether a ligand which is not known to be capable of binding to the 5-HT₂ receptor can bind to the 5-HT₂ receptor, a method for detecting the presence of 5-HT₂ receptor on the surface of a cell, and a method of screening drugs to identify drugs which specifically interact with, and bind to, the 5-HT₂ receptor.

The invention herein also concerns an antibody directed to the human 5-HT₂ receptor, such as a monoclonal antibody directed to an epitope of the 5-HT₂ receptor present on the surface of a cell and having an amino acid sequence included within the amino acid sequence shown in FIG. 2.

Claims

1. An isolated nucleic acid molecule encoding a human 5-HT₂ receptor having the amino acid sequence as shown in FIGS. 2A-2G.

2. A DNA molecule of claim 1.

3. A vector comprising the DNA molecule of claim 2.

4. A virus comprising the DNA molecule of claim 2.

5. A bacteriophage comprising the DNA molecule of claim 2.

6. A plasmid comprising the DNA molecule of claim 2.

7. A mammalian cell comprising the plasmid of claim 6.

8. A cDNA molecule of claim 1.

9. A vector comprising the cDNA molecule of claim 8.

10. A virus comprising the cDNA molecule of claim 8.

11. A bacteriophage comprising the cDNA molecule of claim 8.

12. A plasmid comprising the cDNA molecule of claim 8.

13. The plasmid of claim 12 designated clone 6B.

14. A mammalian cell comprising the plasmid of claim 12.

15. The plasmid designated pMO5-6B.

16. A mammalian cell comprising the plasmid of claim 15.

17. A transfected Ltk- cell comprising the plasmid of claim 15.

18. The transfected Ltk- cell designated L-NGC-5HT₂.