Fluorescent polymer labeled conjugates and intermediates

• US 5,661,040 A
• Filed: 10/14/1994
• Issued: 08/26/1997
• Est. Priority Date: 07/13/1993
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Horng, Wayne J., Bieniarz, Christopher, Huff, Jeffrey B.
• Primary Examiner(s): CEPERLEY, MARY
• Application Number:
  US08/324,004
• Time in Patent Office: 1,047 Days
• Field of Search: 436/532, 436/531, 436/547, 435/6, 435/7.21, 435/7.24, 435/960, 530/391.3
• US Class Current: 436/531
• CPC Class Codes: C08B 37/0012 Cyclodextrin [CD], e.g. cycle with 6 units (alpha), with 7 units (beta) and with 8 units (gamma), large-ring cyclodextrin or cycloamylose with 9 units or more; Derivatives thereof
• CPC Class Codes: G01N 2474/20 Immunohistochemistry assay
• CPC Class Codes: G01N 33/533 with fluorescent label
• CPC Class Codes: G01N 33/582 with fluorescent label

Abstract

The instant invention provides a highly fluorescent conjugate which is useful in specific binding assays, and which comprises a specific binding member bound to a fluorescent polymer. The fluorescent polymer comprises a backbone polymer having multiple signal generating groups immobilized thereon and, optionally, cyclodextrin moieties in association with the polymer. Also provided is a novel process for creating 6-cyclodextrin monoaldehyde.

Claims

1. A method of determining the presence and/or amount of an analyte in a test sample, said method comprising the steps of:

a. forming conjugate/analyte complexes by contacting said test sample with a highly fluorescent conjugate to form a mixture, wherein said conjugate comprises a specific binding member covalently bound to at least one optimized highly-fluorescent-polymer, said highly-fluorescent-polymer comprising a backbone polymer and fluorescent moieties covalently bound to said backbone polymer;

b. separating said conjugate/analyte complexes from said mixture; and

c. detecting a measurable fluorescent signal, wherein the presence and/or amount of said fluorescent signal is related to the amount of said analyte in said test sample, wherein compounds selected from the group consisting of α

-cyclodextrin, β

-cyclodextrin, γ

-cyclodextrin and combinations thereof, are complexed with said fluorescent moieties.

2. A method of determining the presence and/or amount of an analyte in a test sample, said method comprising the steps of:

a. forming conjugate/analyte complexes by contacting said test sample with a highly fluorescent conjugate to form a mixture, wherein said conjugate comprises a specific binding member covalently bound to at least one optimized highly-fluorescent-polymer, said highly-fluorescent-polymer comprising a backbone polymer, cyclodextrin moieties selected from the group consisting of α

-cyclodextrin moieties, β

-cyclodextrin moieties, γ

-cyclodextrin moieties and combinations thereof covalently bound to said backbone polymer, and fluorescent moieties either covalently bound to said backbone polymer or hosted within said cyclodextrin moieties;

b. separating said conjugate/analyte complexes from said mixture; and

c. detecting a measurable fluorescent signal, wherein the presence and/or amount of said fluorescent signal is related to the amount of said analyte in said test sample.

3. The method of claim 2 wherein said specific binding member is an antibody.

4. The method of claim 2 wherein said backbone polymer comprises the residue of an amine functional polymer.

5. The method of claim 4 wherein said amine functional polymer comprises amine functionalities selected from the group consisting of:

a) ##STR8## b) --NH₂, c) ##STR9## wherein R is selected from the group consisting of;

C₁ -C₃ alkyl, isopropyl, (CH₂)₂ CO₂ --, (CH₂)₂ SO₃^-, (CH₂)₂ NH₃⁺, (CH₂)₂ NH₂⁺ (CH₂)₂ SO₃ --(CH₂)₂ O(CH₂)₂ O(CH₂)₂ OH, and (CHOH)₄ CH₂ OH, and

d) combinations thereof.

6. The method of claim 4 wherein said backbone polymer comprises the residue of a polymer selected from the group consisting of:

polyacrylamide hydrazide, polyhydrazide, polylysine and combinations thereof.

7. The method of claim 2 wherein said fluorescent moieties are selected from the group consisting of:

fluorescein, cascade blue, coumarin, Texas Red™ and

phycoerythrin.

8. The method of claim 2 wherein said analyte comprises a nucleic acid sequence.

9. A highly fluorescent conjugate comprising a specific binding member covalently bound to at least one optimized highly-fluorescent-polymer comprising a backbone polymer and fluorescent moieties covalently bound to said backbone polymer, wherein compounds selected from the group consisting of α

-cyclodextrin, β

-cyclodextrin, γ

-cyclodextrin and combinations thereof are complexed with said fluorescent moieties.

10. A highly fluorescent conjugate comprising:

a specific binding member covalently bound to at least one optimized highly-fluorescent-polymer, wherein said highly-fluorescent-polymer comprises a backbone polymer, cyclodextrin moieties selected from the group consisting of α

-cyclodextrin moieties, β

-cyclodextrin moieties, γ

-cyclodextrin moieties and combinations thereof covalently bound to said backbone polymer, and fluorescent moieties, said fluorescent moieties either covalently bound to said backbone polymer or hosted within said cyclodextrin moieties.

11. The conjugate of claim 10 wherein the specific binding member is an antibody.

12. The conjugate of claim 10 wherein said backbone polymer comprises the residue of an amine functional polymer.

13. The conjugate of claim 12 wherein said amine functional polymer comprises amine functionalities selected from the group consisting of:

a) ##STR10## --NH₂, c) ##STR11## wherein R is selected from the group consisting of;

C₁ -C₃ alkyl, isopropyl, --(CH₂)₂ CO₂^-, --(CH₂)₂ SO₃^-, --(CH₂)₂ NH₃⁺, --(CH₂)₂ NH₂⁺ (CH₂)₂ SO₃^-, --(CH₂)₂ O(CH₂)₂ O(CH₂)₂ OH, and --(CHOH)₄ CH₂ OH, and

d) combinations thereof.

14. The conjugate of claim 12 wherein said backbone polymer comprises the residue of a polymer selected from the group consisting of:

polyacrylamide hydrazide, polyhydrazide, polylysine, and combinations thereof.

15. The conjugate of claim 10 wherein said fluorescent moieties are selected from the group consisting of:

fluorescein, cascade blue, coumarin, Texas Red™ and

phycoerythrin.