Method of quatitatively determining one or more characteristics of a substance

• US 5,661,302 A
• Filed: 08/23/1996
• Issued: 08/26/1997
• Est. Priority Date: 08/24/1995
• Status: Expired due to Fees

• Current Assignee: Critikon Co. LLC
• Original Assignee: Johnson & Johnson Medical Incorporated (Johnson & Johnson)
• Inventors: Evans, Peter Dilwyn, Barnett, Nicholas
• Primary Examiner(s): Tokar, Michael J.
• Assistant Examiner(s): Tyler, Virgil O.
• Application Number:
  US08/697,386
• Time in Patent Office: 368 Days
• Field of Search: 250/339.12, 250/339.02, 128/633
• US Class Current: 250/339.12
• CPC Class Codes: A61B 5/14553 specially adapted for cerebral tissue
• CPC Class Codes: G01N 21/359 using near infrared light
• CPC Class Codes: G01N 21/49 within a body or fluid

Abstract

Method of quantitatively determining one or more characteristics of a substance using near infrared spectroscopy. The method including irradiating a point of the substance with radiation at at least two distinct wavelengths, measuring the intensity of the radiation detected at two locations, determining the optical path lengths of the radiation between the irradiation point and the two detecting locations, and determining the effect of the divergence of the radiation reaching two locations. The relative coupling efficiencies of the two detectors are determined by the use of a second emission point equidistant from the two detectors. The characteristic being measured is then determined by the intensity of the radiation detected at the detecting locations with the result modified by accounting for the optical path lengths to the detecting locations, the detector coupling efficiencies and the effect of divergence of the radiation before reaching the detecting locations.

Claims

1. A method of quantitatively determining one or more characteristics of a substance by measurement of radiation absorption spectra of the substance, the method comprising:

i) irradiating a point of the substance with radiation at at least two distinct wavelengths (λ

₁, λ

₂);

ii) measuring the intensity of the radiation (I_t1, I_t2) detected at two locations (D1, D2);

iii) estimating the optical path lengths of the radiation between the irradiation point and the two detecting locations;

iv) determining the effect of the divergence of the radiation reaching the two locations;

v) determining the characteristic based on the intensity of the radiation detected at the detecting locations; and

vi) modifying the result by accounting for the optical path lengths to the detecting locations and the effect of divergence of the radiation before reaching the detecting locations.

2. A method as claimed in claim 1, wherein radiation at a further distinct wavelength is transmitted from a second point equidistant between the detecting locations, the radiation detected at the detecting locations at this wavelength being measured and used to determine the relative gain at the detecting locations.

3. A method as claimed in claim 1, wherein a factor is accounted for when determining the characteristic, the factor relating to radiation absorbers present in the substance other than that of the characteristic being measured and an absorbtion offset is subtracted from the detected radiation intensities.

4. A method as claimed in claim 1, wherein a ratio of the intensities of the detected radiation at the two detecting locations is taken, removing a need for a measure of the attenuation of the radiation at the point of irradiation.

5. A method as claimed in claim 1, wherein a factor is accounted for when determining the characteristic being measured, the factor relating to the relative coupling efficiency of detectors at the detecting locations.

6. A method as claimed in claim 1, wherein the effect of divergence of the radiation detected at the two detecting locations is 1/d², wherein d is the optical path length to each detecting location from the irradiation point.

7. A method as claimed in claim 1, wherein more than one characteristic is measured by using radiation at more than two distinct wavelengths.

8. A method as claimed in claim 1, wherein the substance is part of a human or animal body and the radiation is near infrared radiation.

9. A method as claimed in claim 8, wherein the characteristic is the hemoglobin or cytochrome concentration of the tissue of the human or animal body.

10. A method as claimed in claim 8, wherein the principal tissue component radiation absorber is water.

11. A device for quantitatively determining one or more characteristics of a substance using the method of claim 1.