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Method of sequencing of genoms by hybridization of oligonucleotide probes

  • US 5,667,972 A
  • Filed: 06/05/1995
  • Issued: 09/16/1997
  • Est. Priority Date: 04/01/1987
  • Status: Expired due to Fees
First Claim
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1. A method of determining the sequence of an ambiguous locus in a nucleic acid fragment in a sequencing by hybridization process, said method comprising:

  • (a) prehybridizing said nucleic acid fragment with an excess of unlabeled first oligonucleotide probe which is exactly complementary to one possible sequence at said ambiguous locus;

    (b) competitively hybridizing said nucleic acid fragment with a labeled second oligonucleotide probe which is exactly complementary to a second possible sequence at said ambiguous locus;

    (c) detecting whether the labeled second oligonucleotide probe hybridizes, thereby determining the sequence of said ambiguous locus in said nucleic acid fragment.

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