Electrochemical sensor

• US 5,670,031 A
• Filed: 11/22/1995
• Issued: 09/23/1997
• Est. Priority Date: 06/03/1993
• Status: Expired due to Term

• Current Assignee: Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V.
• Original Assignee: Fraunhofer-Gesellschaft ZUR Forderung Der Angewandten Forschung EV
• Inventors: Schnakenberg, Uwe, Wollenberger, Ulla, Paeschke, Manfred, Hintsche, Rainer
• Primary Examiner(s): Bell, Bruce F.
• Application Number:
  US08/553,266
• Time in Patent Office: 671 Days
• Field of Search: 204/403, 204/412, 204/416, 204/418, 204/409, 422/68.1, 422/82.01, 422/82.02, 422/82.03, 422/98
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01F 1/704 using marked regions or existing inhomogeneities within the fluid stream, e.g. statistically occurring variations in a fluid parameter G01F1/76, G01F25/00 take precedence
• CPC Class Codes: G01N 27/27 Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter
• CPC Class Codes: G01P 5/20 using particles entrained by a fluid stream G01P5/22 takes precedence

Abstract

The invention relates to an electrochemical sensor with interdigital micro-electrodes (1) with structure widths in the sub-μm range. With a twin or multi-pair array of the interdigital micro-electrodes on a substrate (8), the electrochemical detection of molecules with high sensitivity is made possible and further uses for the detection of chemical reaction cycles are made available. The electrodes may be arranged in a micro-channel applied to the substrate having a constant small volume of a few nanoliters. The electrochemical sensor of the invention is suitable for the multiple measurement of the same species of molecule or as a multi-sensitive sensor in chemical analysis and process control in various fields such as biotechnology, environmental protection or health.

Claims

1. An electrochemical sensor, comprising:

substantially dielectric first planar substrate means;

a plurality of micro-electrode means provided on said dielectric substrate means and arranged as an array extending along a set axis, each said micro-electrode means comprising first and second sets of substantially parallel finger means of set lengths and of widths of about 1 μ

m and extending in a direction substantially normal to said axis, said finger means being interdigitatedly arranged and electrically insulated from each other by spacings of about 700 nm;

single reference electrode means provided on said substrate means electrically insulated from said micro-electrode means; and

means for selectively connecting each of said micro-electrode means and said reference electrode means to a different electrical potential.

2. The electrochemical sensor of claim 1, wherein said micro-electrode means comprise thin layers of material of the group including noble metals, potential-forming metal oxides and metal salts inserted into the surface of said substrate in a manner resulting in a planarization of said surface.

3. The electrochemical sensor of claim 2, wherein said plurality of micro-electrode means is located in micro-channel means comprising input and output flow openings, said micro-channel means comprising a groove extending between said input and output means and provided in second substrate means connected in superposition to said first substrate means.

4. The microchemical sensor of claim 3, wherein said micro-channel means comprises mechanical means for immobilizing at least one of chemically and biologically active substances.

5. The microchemical sensor of claim 1, wherein said means for selectively connecting comprises conductor strips extending from said micro-electrode means to contact means and covered by an insulating layer and wherein only said micro-electrode means and said contact means are uncovered thereby to render at least said micro-electrode means openly accessible to analytic media for electrical contacting.

6. The electrochemical sensor of claim 5, wherein said insulating layer comprises silicon oxide.

7. The electrochemical sensor of claim 5, wherein said insulating layer comprises silicon nitride.

8. The electrochemical sensor of claim 1, wherein said plurality of microelectrode means constitute working electrode means.

9. A method of utilizing an electrochemical sensor of the kind comprising substantially dielectric first planar substrate means, a plurality of micro-electrode means provided on said first dielectric substrate means and arranged as an array extending along a set axis and each comprising first and second sets of substantially parallel finger means of set lengths and widths extending in a direction normal to said axis and being interdigitally arranged in electrical insulation from each other, and further comprising reference electrode means on said first substrate means in electrical insulation from said micro-electrode means, and means for connecting said micro-electrode means and said reference electrode means to electrical potential source means for multiple measurement of the same measuring effect, comprising the steps of:

applying a different potential to each of said plurality of micro-electrode means;

subjecting said micro-electrode means to an analyte;

measuring at least one of the amperometric, potentiometric and impedimetric effects at individual micro-electrode means; and

averaging said at least one amperometric, potentiometric, and impedimetric effects at each of said plurality of micro-electrode means for measuring the molecular concentration in a fluid.

10. A method of utilizing an electrochemical sensor of the kind defined in claim 9 for detecting at least one of different chemical reactions and the chronological progress of electrochemical reactions in a given substance, comprising the step of:

subjecting said micro-electrode means to said given substance;

alternatingly applying different potentials to said plurality of micro-electrode means.

11. The method of claim 10, wherein said different potentials are applied simultaneously.

12. The method of claim 10, wherein said different potentials are applied successively.

13. A method of utilizing an electrochemical sensor of the kind defined in claim 9 for measuring at least one of the flow rate on an analytic sample, the change in time of said sample and intermixing effects thereof, comprising the step of:

subjecting said micro-electrode means to said analytic sample;

measuring electrochemically active molecules of said sample at different ones of said microelectrode means by at least one of locally and temporally displaced detection.

14. A method of utilizing an electrochemical sensor of the kind defined in claim 9 for high sensitivity detection of the activity of enzymes of the kind catalyzing the release of an electrochemically reversible substance, comprising the steps of:

subjecting said micro-electrode means to an electrochemically reversible substance;

polarizing one said first and second sets of finger means of at least one of said plurality of micro-electrode means in such a manner that the reduced reaction product of said electrochemically reversible substance is oxidized, and

polarizing the other of the said first and second sets of finger means of said at least one of said plurality of micro-electrode means in such a manner that the oxidized reaction product of said electrochemically reversible substance is reduced.