Antibody to human interleukin-6 receptor

• US 5,670,373 A
• Filed: 12/15/1994
• Issued: 09/23/1997
• Est. Priority Date: 01/22/1988
• Status: Expired due to Term

• Current Assignee: Tadamitsu Kishimoto
• Original Assignee: Tadamitsu Kishimoto
• Inventors: Kishimoto, Tadamitsu
• Primary Examiner(s): Caputa, Anthony C.
• Assistant Examiner(s): NAVARRO, ALBERT MARK
• Application Number:
  US08/357,080
• Time in Patent Office: 1,013 Days
• Field of Search: 435/240.27, 435/172.2, 435/70.21, 435/344, 530/388.23, 530/387.1
• US Class Current: 435/334
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/7155 for interleukins [IL]

Abstract

Antibodies, polyclonal and monoclonal, which are capable of specifically binding to a human interleukin-6 receptor. Also included are monoclonal antibodies which competitively and non-competitively inhibit human interleukin-6, and a method of producing hybridomas of the said monoclonal antibodies capable of specifically binding to a human interleukin-6 receptor.

Claims

1. An isolated antibody to human interleukin-6 receptor, wherein said antibody specifically binds to said human interleukin-6 receptor.

2. An antibody according to claim 1, wherein said antibody can specifically bind to amino acid sequence KTS MHP PYS LGQ LVP of said human interleukin-6 receptor.

3. An antibody according to claim 1, wherein said antibody is monoclonal.

4. An antibody according to claim 3, wherein said antibody is polyclonal.

5. An antibody according to claim 3, wherein said antibody is produced by hybridoma MT18 (FERM BP-2999).

6. An antibody according to claim 3, wherein said antibody is produced by hybridoma PM1 (FERM BP-2998).

7. A hybridoma capable of producing a monoclonal antibody to human interleukin-6 receptor, wherein said monoclonal antibody can specifically bind to human interleukin-6 receptor.

8. A hybridoma according to claim 7, wherein said hybridoma is hybridoma MT18 (FERM BP-2999).

9. A hybridoma according to claim 7, wherein said hybridoma is hybridoma PM1 (FERM BP-2998).

10. A process for the production of a monoclonal antibody to human interleukin-6 receptor, wherein said monoclonal antibody is capable of specifically binding human interleukin-6 receptor, comprising the steps ofculturing hybridoma cells producing said monoclonal antibody;

and

2) recovering the supernatant from said culturing step.

11. A process according to claim 10, wherein said hybridoma cells are hybridoma PM1 cells (FERM BP-2998).

12. A process according to claim 10, wherein said hybridoma cells are hybridoma MT18 cells (FERM BP-2999).

13. A process for the preparation of a polyclonal antibody directed against human interleukin-6 receptor, comprising the steps ofimmunizing an animal with a peptide having the amino acid sequence KTS MHP PYS LGQ LVP;

and

2) recovering said polyclonal antibody from said immunized animal.