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Devices for performing ion-capture binding assays

  • US 5,670,381 A
  • Filed: 05/08/1995
  • Issued: 09/23/1997
  • Est. Priority Date: 01/29/1988
  • Status: Expired due to Fees
First Claim
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1. A test device for determining the presence or amount of an analyte in a test sample, comprising:

  • at least three zones in a porous material;

    a) a first reagent zone containing a diffusive indicator reagent comprising a first specific binding member bound to a detectable label;

    b) a second reagent zone containing a diffusive capture reagent comprising a second specific binding member conjugated to a polymeric ion having a predetermined charge; and

    c) a reaction zone having a pre-determined charge opposite to said polymeric ion charge, whereby said capture reagent and complexes comprising said capture reagent are coupled to the porous material by the ionic attraction of the oppositely charged polymeric ion and porous material;

    wherein said first and second specific binding members are selected from binding members capable of forming a complex with the analyte in a sandwich assay, a competitive assay or an indirect assay, and thereby forming a detectable complex in proportion to the presence or amount of the analyte in the test sample, andwherein said zones may overlap with the proviso that said capture reagent is spatially separated from said reaction zone such that the test sample contacts said capture reagent before contacting said reaction zone.

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