Sugar modified oligonucleotides that detect and modulate gene expression
First Claim
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1. An oligonucleotide that hybridizes with RNA or DNA, having 5 to 50 covalently-bound nucleosides that individually include a ribose or deoxyribose sugar portion and a base portion, wherein:
- said sugar portions of said nucleosides are joined together by 3'"'"'-5'"'"' internucleoside linkages such that the base portions of said nucleosides form a mixed base sequence that is complementary to an RNA base sequence or to a DNA base sequence; and
at least two of said nucleosides include a modified deoxyfuranosyl moiety bearing a 2'"'"'-fluoro substituent; and
whereina duplex formed between said oligonucleotide and its complement exhibits greater thermal stability than does a duplex formed between said complement and an oligonucleotide that does not include 2'"'"'-fluoro substituents.
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Abstract
Compositions and methods are provided for the treatment and diagnosis of diseases amenable to modulation of the production of selected proteins. In accordance with preferred embodiments, oligonucleotides and oligonucleotide analogs are provided which are specifically hybridizable with a selected sequence of RNA or DNA wherein at least two of the 2'"'"'-deoxyfuranosyl moieties of the nucleoside unit is modified. Treatment of HIV, herpes virus, papillomavirus and other infections is provided.
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Citations
3 Claims
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1. An oligonucleotide that hybridizes with RNA or DNA, having 5 to 50 covalently-bound nucleosides that individually include a ribose or deoxyribose sugar portion and a base portion, wherein:
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said sugar portions of said nucleosides are joined together by 3'"'"'-5'"'"' internucleoside linkages such that the base portions of said nucleosides form a mixed base sequence that is complementary to an RNA base sequence or to a DNA base sequence; and at least two of said nucleosides include a modified deoxyfuranosyl moiety bearing a 2'"'"'-fluoro substituent; and
whereina duplex formed between said oligonucleotide and its complement exhibits greater thermal stability than does a duplex formed between said complement and an oligonucleotide that does not include 2'"'"'-fluoro substituents. - View Dependent Claims (2, 3)
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Specification