Highly specific surface for biological reactions having an exposed ethylenic double bond, process of using the surface, and method for assaying for a molecule using the surface

• US 5,677,126 A
• Filed: 02/10/1995
• Issued: 10/14/1997
• Est. Priority Date: 02/11/1994
• Status: Expired due to Term

• Current Assignee: Centre National De La Recherche Scientifique, Institut Pasteur
• Original Assignee: Institut Pasteur
• Inventors: Bensimon, Aaron, Bensimon, David, Heslot, Fran.cedilla.ois
• Primary Examiner(s): Horlick, Kenneth R.
• Application Number:
  US08/386,479
• Time in Patent Office: 977 Days
• Field of Search: 435/6, 435/7.1, 436/94, 436/501, 436/63, 422/104
• US Class Current: 435/6.19
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00628 Ionic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/682 Signal amplification
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2565/518 characterised by the immobilisation of the nucleic acid sample or target
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 33/6803 : General methods of protein analysis not limited to specific proteins or families of proteins
• CPC Class Codes: Y10S 435/969 : Multiple layering of reactants
• CPC Class Codes: Y10S 435/97 : Test strip or test slide
• CPC Class Codes: Y10S 530/81 : Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
• CPC Class Codes: Y10S 530/811 : Peptides or proteins is immobilized on, or in, an inorganic carrier
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/145555 : Hetero-N

Abstract

The present invention relates especially to a highly specific surface for biological reactions, characterized in that it contains a support having at the surface at least one essentially compact layer of an organic compound having, outside the layer, an exposed group containing an ethylenic double bond having affinity for one type of molecule with biological activity under certain reaction conditions, the other elements of the layer being essentially inaccessible for the said molecules under the said reaction conditions.

Claims

1. A process for attaching a nucleic acid on a surface which comprises:

(a) providing a surface having affinity for said nucleic acid and having exposed double bonds;

(b) contacting said surface with said nucleic acid in a fluid at a pH of about 5 to about 6 to attach one end of said nucleic acid to said surface.

2. The process of claim 1, wherein the fluid is a liquid.

3. The process of claim 1, wherein the double bonds are ethylenic double bonds.

4. The process of claim 1, wherein the attachment of said nucleic acid to said surface is by adsorption.

5. The process of claim 1, wherein the nucleic acid is DNA.

6. The process of claim 1, wherein the surface comprises a semiconductor element, an oxide of a semiconductor element, a silicon oxide, or a combination thereof.

7. The product of the process of one of claims 1, 2, 3, 4, 5, or 6.

8. A process for detecting a biological molecule in a sample, comprising:

(a) providing the product of claim 7;

(b) contacting a sample containing a biological molecule with the product of claim 7; and

(c) detecting the presence or absence of binding of the biological molecule to the attached nucleic acid.

9. The process according to claim 8, wherein the biological molecule is DNA, RNA, or protein.