Reagent for determining (1.fwdarw.3)-.beta.-D-glucan
First Claim
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1. A method for producing a reagent for determining (1→
- 3)-β
-D-glucan which comprises treating a limulus amebocyte lysate-containing solution with an adsorbent that specifically adsorbs an endotoxin-sensitive factor, said adsorbent selected from the group consisting of an adsorbent consisting essentially of polyamide and an adsorbent consisting essentially of cellulose, and separating said adsorbent from said treated solution to remove endotoxin-sensitive factors from the limulus amebocyte lysate-containing solution to give a treated limulus amebocyte lysate substantially free from any endotoxin-sensitive factor and containing at least an ingredient capable of specifically reacting with (1→
3)-β
-D-glucan.
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Abstract
The present invention provides a reagent for determining (1→3)-β-D-glucan comprising a lysate substantially free from any endotoxin-sensitive factor, which is obtained by contacting limulus amebocyte lysate optionally containing dextran with a polyamide or cellulose insoluble carrier, which makes it possible to rapidly and easily determine at a high accuracy (1→3)-β-D-glucan of mycotic origin contained in the body fluid such as blood or urine.
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Citations
6 Claims
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1. A method for producing a reagent for determining (1→
- 3)-β
-D-glucan which comprises treating a limulus amebocyte lysate-containing solution with an adsorbent that specifically adsorbs an endotoxin-sensitive factor, said adsorbent selected from the group consisting of an adsorbent consisting essentially of polyamide and an adsorbent consisting essentially of cellulose, and separating said adsorbent from said treated solution to remove endotoxin-sensitive factors from the limulus amebocyte lysate-containing solution to give a treated limulus amebocyte lysate substantially free from any endotoxin-sensitive factor and containing at least an ingredient capable of specifically reacting with (1→
3)-β
-D-glucan. - View Dependent Claims (2, 3, 4, 5, 6)
- 3)-β
Specification