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Systematic evolution of ligands by exponential enrichment: blended SELEX

  • US 5,683,867 A
  • Filed: 04/28/1994
  • Issued: 11/04/1997
  • Est. Priority Date: 06/11/1990
  • Status: Expired due to Term
First Claim
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1. A method for identifying blended nucleic acid ligands of a target compound from a blended candidate mixture comprised of blended nucleic acids wherein each blended nucleic acid comprises a nucleic acid sequence region and a functional unit, wherein said functional unit is a chemical species not naturally associated with nucleic acids and wherein said functional unit imparts at least one of the following functions to the blended nucleic acid ligand:

  • enhanced binding affinity, enhanced membrane partitioning, enhanced membrane permeability, enhanced stability, diagnostic reporting, covalent bond formation, inhibition of biological activity, said method comprising;

    a) contacting the blended candidate mixture with the target, wherein blended nucleic acids having an increased affinity to the target relative to the blended candidate mixture may be partitioned from the remainder of the blended candidate mixture;

    b) partitioning the increased affinity blended nucleic acids from the remainder of the blended candidate mixture; and

    c) amplifying the increased affinity blended nucleic acids to yield a ligand-enriched mixture of blended nucleic acids, whereby blended nucleic acid ligands of the target compound are identified.

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