Apparatus for analyzing cells in urine

• US 5,684,584 A
• Filed: 12/23/1994
• Issued: 11/04/1997
• Est. Priority Date: 05/14/1991
• Status: Expired due to Term

• Current Assignee: Toa Medical Electronics Co., Ltd.
• Original Assignee: Toa Medical Electronics Co., Ltd.
• Inventors: Nakamoto, Hiroyuki, Okada, Tokuhiro
• Primary Examiner(s): Pham, Hoa Q.
• Application Number:
  US08/364,397
• Time in Patent Office: 1,047 Days
• Field of Search: 356/335-343, 356/36, 356/39, 356/73, 356/318, 356/364, 356/243
• US Class Current: 356/336
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 2021/4707 Forward scatter; Low angle scatter

Abstract

The present invention relates to an apparatus for automatically analyzing cells in urine using flow cytometry. The present invention includes means for converting scattered-light signals indicative of individual cells in a urine specimen into corresponding pulse signals, means for converting the pulse width of each pulse signal resulting from the aforementioned conversion into cell-diameter data in accordance with known cell diameters, and means for classifying and enumerating various cells in the urine in correlation with each item of cell-diameter data resulting from the conversion.

Claims

1. Apparatus for analyzing cells in a urine specimen, comprising:

means defining a constricted zone in which cells contained in the urine specimen may flow in single file;

means for irradiating with light the constricted zone;

photoelectric converting circuit for converting light scattered by the cells into an electric signal output;

first-means for converting the electric signal output from said photoelectric converting circuit into pulse-width data;

second means for converting the pulse-width data obtained by the first converting means into cell-diameter data on the basis of cell-diameter values of individual cells measured in advance, wherein the second converting means derives the cell-diameter data solely from the pulse-width data;

cell classifying means for classifying and enumerating the cells in the urine specimen based upon cell judgment values, such that;

(a) a cell is classified as a blood cell when the cell diameter data is 3-15 μ

m;

(b) a cell is classified as an epithelial when the cell diameter data is 15-100 μ

m;

(c) a cell is classified as a cast cell or a epithelial when the cell diameter data is 100-150 μ

m;

(d) a cell is classified as a cast cell when the cell diameter data is greater than 150 μ

m;

(e) a cell is classified as a bacteria when the cell diameter data is 1-3 μ

m; and

means for displaying results of classification and enumeration performed by cell classifying means.

2. The apparatus according to claim 1, the width of the cell flow in the direction of said flow is in the range of 1 μ

m to 20 μ

m.

3. The apparatus according to claim 1, wherein the cell classifying means includes:

means for creating a histogram that is based upon the cell-diameter data; and

means for classifying the histogram into cell regions based upon valleys of the histogram at positions in the vicinity of cell-diameter values of individual cells actually measured in advance.

4. A method of analyzing cells in urine comprising (i) converting scattered light signals indicative of individual cells in a urine specimen into respective pulse signals, (ii) providing cell-diameter data solely from pulse width data by converting the pulse width of each pulse signal into cell-diameter data in accordance with known cell diameter, and (iii) classifying and counting various cells contained in the urine based upon cell judgment, such that

(a) a cell is classified as a blood cell when the cell diameter data is 3-15 μ

m;

(b) a cell is classified as an epithelial when the cell diameter data is 15-100 μ

m;

(c) a cell is classified as a cast cell or an epithelial when the cell diameter data is 100-150 μ

m;

(d) a cell is classified as a cast cell when the cell diameter data is greater than 100 μ

m;

(e) a cell is classified as a bacteria when the cell diameter data is 1-3 μ

m.

5. The method of according to claim 4, the width of the cell flow in the direction of said flow is in the range of 1 μ

m to 20 μ

m.