Brassica vegetable supplement and process for manufacture

• US 5,686,108 A
• Filed: 09/27/1995
• Issued: 11/11/1997
• Est. Priority Date: 09/27/1995
• Status: Expired due to Term

• Current Assignee: Access Business Group International LLC (Alticor Incorporated)
• Original Assignee: Amway Corporation (Alticor Incorporated)
• Inventors: Letiz, Richard E. A., Pusateri, Donald J., Sperrazzo, Phelicia M., Chiang, William C.
• Primary Examiner(s): Page, Thurman K.
• Assistant Examiner(s): Spear, James M.
• Application Number:
  US08/534,645
• Time in Patent Office: 776 Days
• Field of Search: 424/464, 424/489, 424/451
• US Class Current: 424/464
• CPC Class Codes: A23B 7/06 Blanching machines therefor A23N12/00
• CPC Class Codes: A23B 7/155 Microorganisms; Enzymes; Antibiotics
• CPC Class Codes: A23L 19/01 Instant products; Powders; Flakes; Granules A23L19/10 – A23L19/12 take precedence
• CPC Class Codes: A23L 19/10 of tuberous or like starch containing root crops
• CPC Class Codes: A23L 5/25 using enzymes
• CPC Class Codes: C12Y 302/01147 Thioglucosidase (3.2.1.147), i.e. myrosinase

Abstract

A brassica vegetable tablet contains at least 50 ug of sulforaphane and a maximum of 50 ug of sulforaphane-nitrile. A process for producing a brassica vegetable product includes inactivating endogenous myrosinase enzyme in a brassica vegetable and thereafter blending the vegetable with exogenous myrosinase enzyme. The preferred process may include steam blanching as the method of inactivating the endogenous myrosinase enzyme. The preferred process may also include horseradish root as the source of exogenous myrosinase enzyme.

Claims

1. A process for producing a brassica vegetable product with high levels of sulforaphane and low levels of sulforaphane-nitrile comprising the steps of:

(a) deactivating endogenous myrosinase enzyme in a brassica vegetable; and

(b) blending the vegetable with exogenous myrosinase enzyme, wherein the product contains at least about 50 μ

g of sulforaphane and a maximum of about 50 μ

g sulforaphane-nitrile.

2. The process for producing the brassica vegetable product of claim 1 wherein the endogenous myrosinase enzyme is inactivated by steam blanching.

3. The process for producing a brassica vegetable product of claim 1 wherein the brassica vegetable is selected from the group consisting of cabbage, Brussels sprouts, kale, cauliflower, turnip, broccoli, mustard greens, kohlrabi, and horseradish root.

4. The process for producing the brassica vegetable product of claim 1 wherein the brassica vegetable is broccoli.

5. The process for producing the brassica vegetable product of claim 1 wherein the source of exogenous enzyme is a brassica vegetable or mixture thereof.

27. The process for producing the brassica vegetable product of claim 1 wherein the source of exogenous enzyme is horseradish root.

28. The process for producing the brassica vegetable product of claim 1 further comprising the steps of:

(a) preparing a slurry from the blended vegetable and exogenous enzyme;

(b) passing the slurry through a centrifuge;

(c) preparing a powder from the supernatant; and

(d) forming the powder into a tablet.

6. A process for producing a brassica vegetable product with high levels of sulforaphane and low levels of sulforaphane-nitrile comprising the steps of:

(a) deactivating endogenous myrosinase enzyme in a brassica vegetable; and

(b) blending the vegetable with exogenous myrosinase enzyme.

7. The process for producing the brassica product of claim 6 wherein the endogenous myrosinase enzyme is inactivated by steam blanching.

8. The process for producing the brassica vegetable product of claim 6 wherein the brassica vegetable is selected from the group consisting of cabbage, Brussels sprouts, kale, cauliflower, turnip, broccoli, mustard greens, kohlrabi and horseradish root.

9. The process for producing the brassica vegetable product of claim 6 wherein the brassica vegetable is broccoli.

10. The process for producing the brassica vegetable product of claim 6 wherein the source of the exogenous enzyme is a brassica vegetable or mixtures thereof.

11. The process for producing the brassica vegetable product of claim 6 wherein the source of the exogenous enzyme is horseradish root.

12. The process of claim 6 wherein the brassica vegetable product contains at least about 50 ug sulforaphane and a maximum of about 50 ug sulforaphane-nitrile.

13. The process for producing the brassica product of claim 6 further comprising the steps of:

(a) preparing a slurry from the blended vegetable and exogenous enzyme;

(b) passing the slurry through a centrifuge;

(c) preparing a powder from the supernatant; and

(d) forming the powder into a tablet.

14. A process for producing a broccoli tablet with high levels of sulforaphane and low levels of sulforaphane-nitrile comprising the steps of:

(a) steaming fresh broccoli to inactivate endogenous myrosinase enzyme;

(b) blending the blanched broccoli with a source of exogenous myrosinase enzyme;

(c) preparing a slurry from the blended broccoli and exogenous myrosinase enzyme;

(d) passing the slurry through a centrifuge;

(e) forming a powder from the supernatant; and

(f) forming a broccoli tablet from the powder, wherein the tablet contains at least about 50 ug sulforaphane and a maximum of about 50 ug sulforaphane-nitrile.

15. The process recited in claim 14 wherein the steaming is performed at a temperature of about 100°

C.

16. The process of claim 14 wherein the source of exogenous myrosinase enzyme is horseradish root.

17. A process for preparing a broccoli tablet with high levels of sulforaphane and low levels of sulforaphane-nitrile comprising the steps of:

(a) inhibiting conversion of glucoraphanin in fresh broccoli to sulforaphane-nitrile by inactivating myrosinase enzymes in the broccoli;

(b) converting glucoraphanin in fresh broccoli to sulforaphane by adding exogenous myrosinase enzymes; and

(c) preparing a tablet.

18. A brassica vegetable tablet comprising at least about 50 ug by weight of sulforaphane and a maximum of about 50 ug by weight of sulforaphane-nitrile prepared by a process comprising the steps of:

(a) inactivating endogenous myrosinase enzyme in a brassica vegetable; and

(b) blending the vegetable with exogenous myrosinase enzyme.

19. The brassica vegetable tablet prepared by the process of claim 18 wherein the endogenous myrosinase enzyme is inactivated by steam blanching.

20. The brassica vegetable tablet prepared by the process of claim 18 wherein the endogenous myrosinase enzyme is inactivated by immersing the brassica vegetable in ethanol.

21. The brassica vegetable tablet prepared by the process of claim 18 wherein the endogenous myrosinase enzyme is inactivated by water blanching.

22. The brassica vegetable tablet prepared by the process of claim 18 wherein the brassica vegetable is selected from the group consisting of cabbage, Brussels sprouts, kale, cauliflower, turnip, broccoli, mustard greens, kohlrabi and horseradish root.

23. The brassica vegetable tablet prepared by the process of claim 18 wherein the brassica vegetable is broccoli.

24. The brassica vegetable tablet prepared by the process of claim 18 wherein the source of the exogenous enzyme is a brassica vegetable or mixtures thereof.

25. The brassica vegetable tablet prepared by the process of claim 18 wherein the source of the exogenous enzyme is horseradish root.

26. The brassica vegetable tablet prepared by the process of claim 18 comprising the steps of:

(a) preparing a slurry from the blended vegetable and exogenous enzyme;

(b) passing the slurry through a centrifuge;

(c) preparing a powder from the supernatant; and

(d) forming the powder into a tablet.

29. A process for preparing a broccoli tablet with high levels of sulforaphane and low levels of sulforaphane-nitrile comprising the steps of:

(a) inhibiting conversion of glucoraphanin in fresh broccoli to sulforaphane-nitrile by inactivating myrosinase enzymes in the broccoli;

(b) converting glucoraphanin in fresh broccoli to sulforaphane by adding exogenous myrosinase enzymes; and

(c) preparing a tablet, wherein said tablet contains at least about 50 ug sulforaphane and a maximum of about 50 ug of sulforaphane-nitrile.